Keratinocytes as depository of ammonium-inducible glutamine synthetase: age- and anatomy-dependent distribution in human and rat skin.

Details

Serval ID
serval:BIB_DF4140BBDC00
Type
Article: article from journal or magazin.
Collection
Publications
Title
Keratinocytes as depository of ammonium-inducible glutamine synthetase: age- and anatomy-dependent distribution in human and rat skin.
Journal
PloS one
Author(s)
Danielyan L., Zellmer S., Sickinger S., Tolstonog G.V., Salvetter J., Lourhmati A., Reissig D.D., Gleiter C.H., Gebhardt R., Buniatian G.H.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
4
Number
2
Pages
e4416
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
In inner organs, glutamine contributes to proliferation, detoxification and establishment of a mechanical barrier, i.e., functions essential for skin, as well. However, the age-dependent and regional peculiarities of distribution of glutamine synthetase (GS), an enzyme responsible for generation of glutamine, and factors regulating its enzymatic activity in mammalian skin remain undisclosed. To explore this, GS localization was investigated using immunohistochemistry and double-labeling of young and adult human and rat skin sections as well as skin cells in culture. In human and rat skin GS was almost completely co-localized with astrocyte-specific proteins (e.g. GFAP). While GS staining was pronounced in all layers of the epidermis of young human skin, staining was reduced and more differentiated among different layers with age. In stratum basale and in stratum spinosum GS was co-localized with the adherens junction component beta-catenin. Inhibition of, glycogen synthase kinase 3beta in cultured keratinocytes and HaCaT cells, however, did not support a direct role of beta-catenin in regulation of GS. Enzymatic and reverse transcriptase polymerase chain reaction studies revealed an unusual mode of regulation of this enzyme in keratinocytes, i.e., GS activity, but not expression, was enhanced about 8-10 fold when the cells were exposed to ammonium ions. Prominent posttranscriptional up-regulation of GS activity in keratinocytes by ammonium ions in conjunction with widespread distribution of GS immunoreactivity throughout the epidermis allows considering the skin as a large reservoir of latent GS. Such a depository of glutamine-generating enzyme seems essential for continuous renewal of epidermal permeability barrier and during pathological processes accompanied by hyperammonemia.
Keywords
Actins/metabolism, Aging/physiology, Animals, Animals, Newborn, Cell Count, Cells, Cultured, Child, Dexamethasone/pharmacology, Enzyme Induction/drug effects, Glial Fibrillary Acidic Protein/metabolism, Glutamate-Ammonia Ligase/biosynthesis, Humans, Keratinocytes/cytology, Keratinocytes/drug effects, Keratinocytes/enzymology, Lithium Chloride/pharmacology, Male, Metallothionein/metabolism, Protein Transport/drug effects, Quaternary Ammonium Compounds/pharmacology, Rats, Scalp/cytology, Scalp/drug effects, Scalp/metabolism, Skin/anatomy & histology, Skin/cytology, Skin/drug effects, Skin/enzymology, beta Catenin/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
15/12/2017 17:07
Last modification date
14/01/2020 7:26
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