Generation of tumor-targeted human T lymphocytes from induced pluripotent stem cells for cancer therapy.

Détails

ID Serval
serval:BIB_DF31C893F59B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Generation of tumor-targeted human T lymphocytes from induced pluripotent stem cells for cancer therapy.
Périodique
Nature biotechnology
Auteur(s)
Themeli M., Kloss C.C., Ciriello G., Fedorov V.D., Perna F., Gonen M., Sadelain M.
ISSN
1546-1696 (Electronic)
ISSN-L
1087-0156
Statut éditorial
Publié
Date de publication
10/2013
Peer-reviewed
Oui
Volume
31
Numéro
10
Pages
928-933
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Résumé
Progress in adoptive T-cell therapy for cancer and infectious diseases is hampered by the lack of readily available, antigen-specific, human T lymphocytes. Pluripotent stem cells could provide an unlimited source of T lymphocytes, but the therapeutic potential of human pluripotent stem cell-derived lymphoid cells generated to date remains uncertain. Here we combine induced pluripotent stem cell (iPSC) and chimeric antigen receptor (CAR) technologies to generate human T cells targeted to CD19, an antigen expressed by malignant B cells, in tissue culture. These iPSC-derived, CAR-expressing T cells display a phenotype resembling that of innate γδ T cells. Similar to CAR-transduced, peripheral blood γδ T cells, the iPSC-derived T cells potently inhibit tumor growth in a xenograft model. This approach of generating therapeutic human T cells 'in the dish' may be useful for cancer immunotherapy and other medical applications.
Mots-clé
Animals, Antigens, CD19/metabolism, Cell Differentiation, Cell Proliferation, Chromium Radioisotopes, Cluster Analysis, Humans, Induced Pluripotent Stem Cells/cytology, Male, Mice, Neoplasms/immunology, Neoplasms/therapy, Phenotype, Protein Engineering, Receptors, Antigen, T-Lymphocytes/cytology, T-Lymphocytes/immunology
Pubmed
Web of science
Création de la notice
06/07/2018 11:02
Dernière modification de la notice
20/08/2019 16:03
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