Cloning, localization and induction of mouse brain glycogen synthase

Details

Serval ID
serval:BIB_DE52042F589B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cloning, localization and induction of mouse brain glycogen synthase
Journal
Brain Research. Molecular Brain Research
Author(s)
Pellegri  G., Rossier  C., Magistretti  P. J., Martin  J. L.
ISSN
0169-328X
Publication state
Published
Issued date
06/1996
Peer-reviewed
Oui
Volume
38
Number
2
Pages
191-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
The cDNA for mouse brain glycogen synthase has been isolated by screening a mouse cerebral cortical astrocyte lambda ZAP II cDNA library. The mouse brain glycogen synthase cDNA is 3.5 kilobases in length and encodes a protein of 737 amino acids. The coding sequence of mouse brain glycogen synthase cDNA shares approximately 87% nucleotide identity and approximately 96% amino acid identity with the muscle isozyme, while the degree of identity is lower with the liver isozyme. The regional distribution of glycogen synthase mRNA determined by in situ hybridization in the mouse brain reveals a wide distribution throughout the central nervous system with highest densities observed in the cerebellum, hippocampus and olfactory bulb. At the cellular level the expression of brain glycogen synthase mRNA is localized both in astrocytes and neurons with, however, the higher levels observed in astrocytes. Vasoactive intestinal peptide and noradrenaline, two neurotransmitters previously shown to induce a glycogen resynthesis in cultured astrocytes, upregulate the expression of glycogen synthase mRNA in this cell type but not in neurons.
Keywords
Amino Acid Sequence Animals Astrocytes/*enzymology Base Sequence Cells, Cultured Cerebral Cortex/cytology/*enzymology Cloning, Molecular DNA, Complementary/*isolation & purification Enzyme Induction Genetic Code Glycogen Synthase/analysis/biosynthesis/*genetics Mice Mice, Inbred Strains Molecular Sequence Data Norepinephrine/pharmacology RNA, Messenger/analysis Vasoactive Intestinal Peptide/pharmacology
Pubmed
Create date
20/01/2008 19:22
Last modification date
20/08/2019 17:02
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