A molecular and neuronal basis for amino acid sensing in the Drosophila larva.

Détails

Ressource 1Télécharger: srep34871.pdf (1467.92 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_DD8C9FAFF6EF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A molecular and neuronal basis for amino acid sensing in the Drosophila larva.
Périodique
Scientific reports
Auteur(s)
Croset V., Schleyer M., Arguello J.R., Gerber B., Benton R.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
16/12/2016
Peer-reviewed
Oui
Volume
6
Pages
34871
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Amino acids are important nutrients for animals, reflected in conserved internal pathways in vertebrates and invertebrates for monitoring cellular levels of these compounds. In mammals, sensory cells and metabotropic glutamate receptor-related taste receptors that detect environmental sources of amino acids in food are also well-characterised. By contrast, it is unclear how insects perceive this class of molecules through peripheral chemosensory mechanisms. Here we investigate amino acid sensing in Drosophila melanogaster larvae, which feed ravenously to support their rapid growth. We show that larvae display diverse behaviours (attraction, aversion, neutral) towards different amino acids, which depend upon stimulus concentration. Some of these behaviours require IR76b, a member of the variant ionotropic glutamate receptor repertoire of invertebrate chemoreceptors. IR76b is broadly expressed in larval taste neurons, suggesting a role as a co-receptor. We identify a subpopulation of these neurons that displays physiological activation by some, but not all, amino acids, and which mediate suppression of feeding by high concentrations of at least a subset of these compounds. Our data reveal the first elements of a sophisticated neuronal and molecular substrate by which these animals detect and behave towards external sources of amino acids.
Mots-clé
Amino Acids/chemistry, Animals, Behavior, Animal/physiology, Brain/pathology, Calcium/metabolism, Drosophila/growth & development, Drosophila/metabolism, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Larva/metabolism, Mutagenesis, Neurons/metabolism, Receptors, Ionotropic Glutamate/genetics, Receptors, Ionotropic Glutamate/metabolism, Sodium Channels/genetics, Sodium Channels/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/12/2016 13:14
Dernière modification de la notice
20/08/2019 16:02
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