Chromosome remodelling by SMC/Condensin in B. subtilis is regulated by monomeric Soj/ParA during growth and sporulation.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_DD6E45BF5EF5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Chromosome remodelling by SMC/Condensin in B. subtilis is regulated by monomeric Soj/ParA during growth and sporulation.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Roberts D.M., Anchimiuk A., Kloosterman T.G., Murray H., Wu L.J., Gruber S., Errington J.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
11/10/2022
Peer-reviewed
Oui
Volume
119
Number
41
Pages
e2204042119
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
SMC complexes, loaded at ParB-parS sites, are key mediators of chromosome organization in bacteria. ParA/Soj proteins interact with ParB/Spo0J in a pathway involving adenosine triphosphate (ATP)-dependent dimerization and DNA binding, facilitating chromosome segregation in bacteria. In Bacillus subtilis, ParA/Soj also regulates DNA replication initiation and along with ParB/Spo0J is involved in cell cycle changes during endospore formation. The first morphological stage in sporulation is the formation of an elongated chromosome structure called an axial filament. Here, we show that a major redistribution of SMC complexes drives axial filament formation in a process regulated by ParA/Soj. Furthermore, and unexpectedly, this regulation is dependent on monomeric forms of ParA/Soj that cannot bind DNA or hydrolyze ATP. These results reveal additional roles for ParA/Soj proteins in the regulation of SMC dynamics in bacteria and yet further complexity in the web of interactions involving chromosome replication, segregation and organization, controlled by ParAB and SMC.
Keywords
Adenosine Triphosphatases, Adenosine Triphosphate/metabolism, Bacillus subtilis/metabolism, Bacterial Proteins/metabolism, Chromosome Segregation, Chromosomes, Bacterial/genetics, Chromosomes, Bacterial/metabolism, DNA, Bacterial/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Multiprotein Complexes, B. subtilis, ParABS, SMC/Condensin, chromosome segregation, sporulation
Pubmed
Open Access
Yes
Create date
18/10/2022 8:43
Last modification date
23/01/2024 7:35
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