Tissue-specific transgenic and knockout mice

Détails

ID Serval
serval:BIB_DD6C4C34ED92
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Tissue-specific transgenic and knockout mice
Périodique
Methods in Molecular Biology
Auteur(s)
Porret A., Mérillat A. M., Guichard S., Beermann F., Hummler E.
ISSN
1064-3745 (Print)
Statut éditorial
Publié
Date de publication
2006
Volume
337
Pages
185-205
Notes
Journal Article Research Support, Non-U.S. Gov't
Résumé
Analysis of genetically engineered mice is crucial for our understanding of the in vivo function of genes and proteins in the whole organism. This includes inactivation of a gene or the generation of specific mutations. The development of knockout and transgenic technologies in the mouse, therefore, represents a powerful tool for elucidating gene function, for modeling of human diseases, and potentially for the evaluation of drugs. In particular, conditional gene targeting applying the Cre/loxP-mediated recombination system is increasingly used to evaluate the role of the gene of interest in a cell-type-specific or even inducible manner. The experimental steps start with the characterization of the gene locus, followed by construction of a vector, gene targeting in ES cells, and establishment of mouse lines carrying the desired mutation. These are then bred to transgenic mice expressing Cre recombinase in a tissue-specific manner, thus allowing gene inactivation in a cell type of interest.
Mots-clé
Animals Chimera/genetics Embryo/cytology Female *Gene Deletion Genetic Vectors Humans Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic *Organ Specificity Stem Cells/metabolism Transfection Transgenes/*genetics
Pubmed
Création de la notice
24/01/2008 13:42
Dernière modification de la notice
20/08/2019 17:02
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