Clonal expansion of T cells: a cytotoxic T-cell response in vivo that involves precursor cell proliferation.

Détails

ID Serval
serval:BIB_DCC3C2E897DC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Clonal expansion of T cells: a cytotoxic T-cell response in vivo that involves precursor cell proliferation.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Denizot F., Wilson A., Battye F., Berke G., Shortman K.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
1986
Volume
83
Numéro
16
Pages
6089-6092
Langue
anglais
Résumé
The response of peritoneal exudate lymphocytes to allogeneic tumor cells was used to determine whether the in vivo generation of cytotoxic T cells (CTL) involved the proliferation of precursor cells. Ten days post-injection, both cytotoxic activity and the formation of conjugates between lymphocytes and target cells were shown to be specific for the immunizing tumor alloantigens and to be effected by Ly-2+ cells. A cell-sorting-based procedure was developed to isolate specific conjugates between red-fluorescence-tagged CTL and blue-fluorescence-tagged tumor target cells. When [3H]thymidine was administered during the response, almost all isolated conjugate-forming CTL were 3H-labeled on autoradiography. Thus, the CTL were clearly products of dividing cells, a result that contradicts published data. Reassessment of a previously studied system, which suggested that CTL were not products of cell division, indicated that in that system many of the conjugate-forming cytotoxic cells studied were Ly-2- and nonspecific, and thus perhaps not T cells. We conclude that the clonal selection model is applicable to at least one in vivo T-cell response.
Mots-clé
Animals, Cell Division, Cell Line, Clone Cells, Cytotoxicity, Immunologic, DNA Replication, Lymphocyte Activation, Lymphoma/immunology, Mast-Cell Sarcoma/immunology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred CBA, Phenotype, T-Lymphocytes, Cytotoxic/immunology, Thymidine/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/04/2013 12:41
Dernière modification de la notice
20/08/2019 16:01
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