Generation and immunogenicity of novel HIV/AIDS vaccine candidates targeting HIV-1 Env/Gag-Pol-Nef antigens of clade C

Details

Serval ID
serval:BIB_DC952B01F704
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Generation and immunogenicity of novel HIV/AIDS vaccine candidates targeting HIV-1 Env/Gag-Pol-Nef antigens of clade C
Journal
Vaccine
Author(s)
Gomez  C. E., Najera  J. L., Jimenez  V., Bieler  K., Wild  J., Kostic  L., Heidari  S., Chen  M., Frachette  M. J., Pantaleo  G., Wolf  H., Liljestrom  P., Wagner  R., Esteban  M.
ISSN
0264-410X (Print)
Publication state
Published
Issued date
03/2007
Volume
25
Number
11
Pages
1969-92
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 1
Abstract
Recombinants based on the attenuated vaccinia virus strains MVA and NYVAC are considered candidate vectors against different human diseases. In this study we have generated and characterized in BALB/c and in transgenic HHD mice the immunogenicity of two attenuated poxvirus vectors expressing in a single locus (TK) the codon optimized HIV-1 genes encoding gp120 and Gag-Pol-Nef (GPN) polyprotein of clade C (referred as MVA-C and NYVAC-C). In HHD mice primed with either MVA-C or NYVAC-C, or primed with DNA-C and boosted with the poxvirus vectors, the splenic T cell responses against clade C peptides spanning gp120/GPN was broad and mainly directed against Gag-1, Env-1 and Env-2 peptide pools. In BALB/c mice immunized with the homologous or the heterologous combination of poxvirus vectors or with Semliki forest virus (SFV) vectors expressing gp120/GPN, the immune response was also broad but the most immunogenic peptides were Env-1, GPN-1 and GPN-2. Differences in the magnitude of the cellular immune responses were observed between the poxvirus vectors depending on the protocol used. The specific cellular immune response triggered by the poxvirus vectors was Th1 type. The cellular response against the vectors was higher for NYVAC than for MVA in both HHD and BALB/c mice, but differences in viral antigen recognition between the vectors was observed in sera from the poxvirus-immunized animals. These results demonstrate the immunogenic potential of MVA-C and NYVAC-C as novel vaccine candidates against clade C of HIV-1.
Keywords
AIDS Vaccines/genetics/*immunology Animals Base Sequence Codon/genetics Gene Products, gag/genetics/immunology Gene Products, nef/genetics/immunology Gene Products, pol/genetics/immunology Genetic Vectors HIV Antigens/genetics/*immunology HIV Envelope Protein gp120/genetics/immunology HIV-1/genetics/*immunology Humans Immunization, Secondary Interferon Type II/biosynthesis Interleukin-2/biosynthesis Mice Mice, Inbred BALB C Mice, Transgenic Models, Animal Molecular Sequence Data Semliki forest virus Spleen/immunology T-Lymphocytes/immunology Vaccines, DNA/genetics/immunology Vaccinia virus Viral Vaccines
Pubmed
Web of science
Create date
25/01/2008 15:14
Last modification date
20/08/2019 16:01
Usage data