Evidence for a new post-translational modification in Staphylococcus aureus: Hydroxymethylation of asparagine and glutamine.

Détails

ID Serval
serval:BIB_DB18CCA01E89
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Evidence for a new post-translational modification in Staphylococcus aureus: Hydroxymethylation of asparagine and glutamine.
Périodique
Journal of Proteomics
Auteur(s)
Waridel P., Ythier M., Gfeller A., Moreillon P., Quadroni M.
ISSN
1876-7737 (Electronic)
Statut éditorial
Publié
Date de publication
2012
Volume
75
Numéro
6
Pages
1742-1751
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Staphylococcus aureus is an opportunistic pathogen whose infectious capacity depends on surface proteins, which enable bacteria to colonize and invade host tissues and cells. We analyzed "trypsin-shaved" surface proteins of S. aureus cultures by high resolution LC-MS/MS at different growth stages and culture conditions. Some modified peptides were identified, with a mass shift corresponding to the addition of a CH(2)O group (+30.0106u). We present evidence that this shift corresponds to a hyxdroxymethylation of asparagine and glutamine residues. This known but poorly documented post-translational modification was only found in a few proteins of S. aureus grown under specific conditions. This specificity seemed to exclude the hypothesis of an artifact due to sample preparation. Altogether hydroxymethylation was observed in 35 peptides from 15 proteins in our dataset, which corresponded to 41 modified sites, 35 of them being univocally localized. While no function can currently be assigned to this post-translational modification, we hypothesize that it could be linked to modulation of virulence factors, since it was mostly found on some surface proteins of S. aureus.
Pubmed
Web of science
Création de la notice
12/01/2012 16:00
Dernière modification de la notice
20/08/2019 16:00
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