Role of dendritic cells in the immune response induced by mouse mammary tumor virus superantigen.

Détails

ID Serval
serval:BIB_DAD6C5827009
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Role of dendritic cells in the immune response induced by mouse mammary tumor virus superantigen.
Périodique
Journal of Virology
Auteur(s)
Baribaud F., Maillard I., Vacheron S., Brocker T., Diggelmann H., Acha-Orbea H.
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
73
Numéro
10
Pages
8403-8410
Langue
anglais
Résumé
After mouse mammary tumor virus (MMTV) infection, B lymphocytes present a superantigen (Sag) and receive help from the unlimited number of CD4(+) T cells expressing Sag-specific T-cell receptor Vbeta elements. The infected B cells divide and differentiate, similarly to what occurs in classical B-cell responses. The amplification of Sag-reactive T cells can be considered a primary immune response. Since B cells are usually not efficient in the activation of naive T cells, we addressed the question of whether professional antigen-presenting cells such as dendritic cells (DCs) are responsible for T-cell priming. We show here, using MMTV(SIM), a viral isolate which requires major histocompatibility complex class II I-E expression to induce a strong Sag response in vivo, that transgenic mice expressing I-E exclusively on DCs (I-EalphaDC tg) reveal a strong Sag response. This Sag response was dependent on the presence of B cells, as indicated by the absence of stimulation in I-EalphaDC tg mice lacking B cells (I-EalphaDC tg muMT(-/-)), even if these B cells lack I-E expression. Furthermore, the involvement of either residual transgene expression by B cells or transfer of I-E from DCs to B cells was excluded by the use of mixed bone marrow chimeras. Our results indicate that after priming by DCs in the context of I-E, the MMTV(SIM) Sag can be recognized on the surface of B cells in the context of I-A. The most likely physiological relevance of the lowering of the antigen threshold required for T-cell/B-cell collaboration after DC priming is to allow B cells with a low affinity for antigen to receive T-cell help in a primary immune response.
Mots-clé
Animals, Antigen Presentation, Antigens, Viral/immunology, Dendritic Cells/immunology, Female, Immunity, Cellular, Mammary Tumor Virus, Mouse/immunology, Mice, Mice, Inbred C57BL, Retroviridae Infections/immunology, Superantigens/immunology, Tumor Virus Infections/immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:48
Dernière modification de la notice
03/03/2018 21:55
Données d'usage