Effects of tumor promoters on growth and on cellular redistribution of phospholipid/Ca2+-dependent protein kinase in human breast cancer cells

Détails

ID Serval
serval:BIB_DAC5A5FD728C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Effects of tumor promoters on growth and on cellular redistribution of phospholipid/Ca2+-dependent protein kinase in human breast cancer cells
Périodique
International Journal of Cancer
Auteur(s)
Regazzi  R., Fabbro  D., Costa  S. D., Borner  C., Eppenberger  U.
ISSN
0020-7136 (Print)
Statut éditorial
Publié
Date de publication
05/1986
Volume
37
Numéro
5
Pages
731-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 15
Résumé
Active, structurally unrelated tumor promoters (12-0-tetradecanoyl-phorbol-13-acetate (TPA), teleocidin and aplysiatoxin) inhibit growth of mammary carcinoma cells (MCF7- greater than BT-20 greater than MDA-MB-231 greater than = ZR-75-1 greater than HBL-100). This efficiency in inhibiting cell growth correlates with the tumor-promoting activity of a series of phorbol ester derivatives. The phospholipid/calcium-dependent protein kinase (PKC), a target for phorbol ester action, was measured by polyacrylamide gel electrophoresis. The levels of PKC were higher (p less than 0.001) in estrogen-receptor-negative than in estrogen-receptor-positive cells. Treatment of cells with active tumor promoters results in time- and dose-dependent translocation of cytosolic PKC to membrane fractions. Less potent phorbol esters induce only partial translocation of PKC (i.e., decrease of cytosolic without increase in membrane-bound PKC), whereas inactive esters have no effect. No correlation was found between PKC concentration or the amount of PKC translocated to membranes and the sensitivity of the respective cells to TPA. It is concluded that tumor-promoter-mediated growth inhibition of breast cancer cell lines is due to mechanism(s) occurring after the translocation of PKC.
Mots-clé
Breast Neoplasms/enzymology/*pathology Carcinogens/*pharmacology Cell Compartmentation/drug effects Cell Cycle/drug effects Cytosol/enzymology Dose-Response Relationship, Drug Female Humans Lyngbya Toxins/pharmacology Membranes/enzymology Protein Kinase C/*metabolism Receptors, Estrogen/metabolism Tetradecanoylphorbol Acetate/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 15:30
Dernière modification de la notice
03/03/2018 21:55
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