PPARγ Controls Ectopic Adipogenesis and Cross-Talks with Myogenesis During Skeletal Muscle Regeneration.

Détails

Ressource 1Télécharger: ijms-19-02044-v2.pdf (2409.23 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_DA3296240EF0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
PPARγ Controls Ectopic Adipogenesis and Cross-Talks with Myogenesis During Skeletal Muscle Regeneration.
Périodique
InternationalJjournal of Molecular Sciences
Auteur(s)
Dammone G., Karaz S., Lukjanenko L., Winkler C., Sizzano F., Jacot G., Migliavacca E., Palini A., Desvergne B., Gilardi F., Feige J.N.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
19
Numéro
7
Pages
2044
Langue
anglais
Résumé
Skeletal muscle is a regenerative tissue which can repair damaged myofibers through the activation of tissue-resident muscle stem cells (MuSCs). Many muscle diseases with impaired regeneration cause excessive adipose tissue accumulation in muscle, alter the myogenic fate of MuSCs, and deregulate the cross-talk between MuSCs and fibro/adipogenic progenitors (FAPs), a bi-potent cell population which supports myogenesis and controls intra-muscular fibrosis and adipocyte formation. In order to better characterize the interaction between adipogenesis and myogenesis, we studied muscle regeneration and MuSC function in whole body <i>Pparg</i> null mice generated by epiblast-specific Cre/lox deletion ( <i>Pparg <sup>Δ/Δ</sup></i> ). We demonstrate that deletion of PPARγ completely abolishes ectopic muscle adipogenesis during regeneration and impairs MuSC expansion and myogenesis after injury. Ex vivo assays revealed that perturbed myogenesis in <i>Pparg <sup>Δ/Δ</sup></i> mice does not primarily result from intrinsic defects of MuSCs or from perturbed myogenic support from FAPs. The immune transition from a pro- to anti-inflammatory MuSC niche during regeneration is perturbed in <i>Pparg <sup>Δ/Δ</sup></i> mice and suggests that PPARγ signaling in macrophages can interact with ectopic adipogenesis and influence muscle regeneration. Altogether, our study demonstrates that a PPARγ-dependent adipogenic response regulates muscle fat infiltration during regeneration and that PPARγ is required for MuSC function and efficient muscle repair.
Mots-clé
PPARg, Skeletal muscle, adipogenesis, inflammation, muscle stem cells, myogenesis, regeneration, satellite cells
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/09/2018 7:27
Dernière modification de la notice
20/08/2019 15:59
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