Perforin and its role in T lymphocyte-mediated cytolysis

Détails

ID Serval
serval:BIB_D9DB6EE712A6
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Perforin and its role in T lymphocyte-mediated cytolysis
Périodique
Experientia
Auteur(s)
Lowin  B., Krahenbuhl  O., Muller  C., Dupuis  M., Tschopp  J.
ISSN
0014-4754 (Print)
Statut éditorial
Publié
Date de publication
10/1992
Volume
48
Numéro
10
Pages
911-20
Notes
Journal Article
Review --- Old month value: Oct 15
Résumé
The killing mediated by cytotoxic T lymphocytes (CTL) represents an important mechanism in the immune defence against tumors and virus infections. The lytic mechanism has been proposed to consist of a polarized secretion of granule-stored molecules, occurring on effector-target cell contact. By electron microscopy, membrane deposited, pore-like lesions are detected on the target cell membrane during cytolysis by CTL. These structures resembled strikingly pores formed during complement attack. Granules of CTL isolated by nitrogen cavitation and Percoll gradient centrifugation were shown to retain cytotoxic activity. Further purification of proteins stored in these granules led to the discovery of a membranolytic protein named perforin which was capable of polymerizing into pore-like structures. In addition to this cytolytic protein, a set of serine esterases was found as well as lysosomal enzymes and proteoglycans, whose function are not yet clearly defined. The role of perforin in the cytotoxic process is currently being explored by ablating the active gene in mice.
Mots-clé
Animals Antibody-Dependent Cell Cytotoxicity *Cytotoxicity, Immunologic Exons Humans Killer Cells, Natural/*immunology Membrane Glycoproteins/genetics/*physiology Models, Biological Pore Forming Cytotoxic Proteins T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Création de la notice
24/01/2008 16:18
Dernière modification de la notice
03/03/2018 21:53
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