Antifungal activity against planktonic and biofilm Candida albicans in an experimental model of foreign-body infection.

Détails

ID Serval
serval:BIB_D9CD5F5F310B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Antifungal activity against planktonic and biofilm Candida albicans in an experimental model of foreign-body infection.
Périodique
The Journal of infection
Auteur(s)
Maiolo E.M., Oliva A., Furustrand Tafin U., Perrotet N., Borens O., Trampuz A.
ISSN
1532-2742 (Electronic)
ISSN-L
0163-4453
Statut éditorial
Publié
Date de publication
03/2016
Peer-reviewed
Oui
Volume
72
Numéro
3
Pages
386-392
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication types: Journal Article ; Research Support, Non-U.S. Gov't

Résumé
The treatment of Candida implant-associated infections remains challenging. We investigated the antifungal activity against planktonic and biofilm Candida albicans in a foreign-body infection model.
Teflon cages were subcutaneously implanted in guinea pigs, infected with C. albicans (ATCC 90028). Animals were treated intraperitoneally 12 h after infection for 4 days once daily with saline, fluconazole (16 mg/kg), amphotericin B (2.5 mg/kg), caspofungin (2.5 mg/kg) or anidulafungin (20 mg/kg). Planktonic Candida was quantified, the clearance rate and cure rate determined.
In untreated animals, planktonic Candida was cleared from cage fluid in 25% (infected with 4.5 × 10(3) CFU/cage), 8% (infected with 4.8 × 10(4) CFU/cage) and 0% (infected with 6.2 × 10(5) CFU/cage). Candida biofilm persisted on all explanted cages. Compared to untreated controls, caspofungin reduced the number of planktonic C. albicans to 0.22 and 0.0 CFU/ml, respectively, and anidulafungin to 0.11 and 0.13 CFU/ml, respectively. Fluconazole cured 2/12 cages (17%), amphotericin B and anidulafungin 1/12 cages (8%) and caspofungin 3/12 cages (25%).
Echinocandins showed superior activity against planktonic C. albicans. Caspofungin showed the highest cure rate of C. albicans biofilm. However, no antifungal exceeded 25% cure rate, demonstrating the difficulty of eradicating Candida biofilms from implants.

Mots-clé
Animals, Antifungal Agents/administration & dosage, Antifungal Agents/pharmacology, Biofilms/drug effects, Candida albicans/drug effects, Candida albicans/physiology, Candidiasis/drug therapy, Candidiasis/microbiology, Colony Count, Microbial, Disease Models, Animal, Foreign Bodies/complications, Guinea Pigs, Injections, Intraperitoneal, Male, Treatment Outcome
Pubmed
Création de la notice
25/01/2016 13:33
Dernière modification de la notice
24/04/2019 17:00
Données d'usage