Cloned transcription factor MTF-1 activates the mouse metallothionein I promoter

Détails

ID Serval
serval:BIB_D9C73D51DD1E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cloned transcription factor MTF-1 activates the mouse metallothionein I promoter
Périodique
EMBO Journal
Auteur(s)
Radtke  F., Heuchel  R., Georgiev  O., Hergersberg  M., Gariglio  M., Dembic  Z., Schaffner  W.
ISSN
0261-4189 (Print)
Statut éditorial
Publié
Date de publication
04/1993
Volume
12
Numéro
4
Pages
1355-62
Notes
Journal Article --- Old month value: Apr
Résumé
Metallothioneins (MTs) are small cysteine-rich proteins whose structure is conserved from fungi to man. MTs strongly bind heavy metals, notably zinc, copper and cadmium. Upon exposure of cells to heavy metal and other adverse treatments, MT gene transcription is strongly enhanced. Metal induction is mediated by several copies of a 15 bp consensus sequence (metal-responsive element, MRE) present in the promoter region of MT genes. We and others have demonstrated the presence of an MRE-binding factor in HeLa cell nuclear extracts. We found that this factor, termed MTF-1 (MRE-binding transcription factor) is inactivated/reactivated in vitro by zinc withdrawal/addition. Here we report that the amounts of MTF-1-DNA complexes are elevated several-fold in zinc-treated cells, as measured by bandshift assay. We have also cloned the cDNA of mouse MTF-1, a 72.5 kDa protein. MTF-1 contains six zinc fingers and separate transcriptional activation domains with high contents of acidic and proline residues. Ectopic expression of MTF-1 in primate or rodent cells strongly enhances transcription of a reporter gene that is driven by four consensus MREd sites, or by the complete mouse MT-I promoter, even at normal zinc levels.
Mots-clé
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA-Binding Proteins/genetics Gene Expression Metallothionein/*genetics Mice Molecular Sequence Data Oligodeoxyribonucleotides/chemistry/metabolism *Promoter Regions (Genetics) RNA, Messenger/genetics Recombinant Proteins/metabolism Transcription Factors/*genetics Zinc/physiology Zinc Fingers
Pubmed
Web of science
Création de la notice
28/01/2008 12:39
Dernière modification de la notice
03/03/2018 21:53
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