En face optical coherence tomography of foveal microstructure in full-thickness macular hole: a model to study perifoveal müller cells.

Détails

Ressource 1Télécharger: BIB_D992C0F6B42C.P001.pdf (1067.60 [Ko])
Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_D992C0F6B42C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
En face optical coherence tomography of foveal microstructure in full-thickness macular hole: a model to study perifoveal müller cells.
Périodique
American Journal of Ophthalmology
Auteur(s)
Matet A., Savastano M.C., Rispoli M., Bergin C., Moulin A., Crisanti P., Behar-Cohen F., Lumbroso B.
ISSN
1879-1891 (Electronic)
ISSN-L
0002-9394
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
159
Numéro
6
Pages
1142-1151.e3
Langue
anglais
Résumé
PURPOSE: To characterize perifoveal intraretinal cavities observed around full-thickness macular holes (MH) using en face optical coherence tomography and to establish correlations with histology of human and primate maculae.
DESIGN: Retrospective nonconsecutive observational case series.
METHODS: Macular en face scans of 8 patients with MH were analyzed to quantify the areas of hyporeflective spaces, and were compared with macular flat mounts and sections from 1 normal human donor eye and 2 normal primate eyes (Macaca fascicularis). Immunohistochemistry was used to study the distribution of glutamine synthetase, expressed by Müller cells, and zonula occludens-1, a tight-junction protein.
RESULTS: The mean area of hyporeflective spaces was lower in the inner nuclear layer (INL) than in the complex formed by the outer plexiform (OPL) and the Henle fiber layers (HFL): 5.0 × 10(-3) mm(2) vs 15.9 × 10(-3) mm(2), respectively (P < .0001, Kruskal-Wallis test). In the OPL and HFL, cavities were elongated with a stellate pattern, whereas in the INL they were rounded and formed vertical cylinders. Immunohistochemistry confirmed that Müller cells followed a radial distribution around the fovea in the frontal plane and a "Z-shaped" course in the axial plane, running obliquely in the OPL and HFL and vertically in the inner layers. In addition, zonula occludens-1 co-localized with Müller cells within the complex of OPL and HFL, indicating junctions in between Müller cells and cone axons.
CONCLUSION: The dual profile of cavities around MHs correlates with Müller cell morphology and is consistent with the hypothesis of intra- or extracellular fluid accumulation along these cells.
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/06/2015 9:18
Dernière modification de la notice
09/05/2019 2:01
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