The HIV-1 inducer of short transcripts activates the synthesis of 5,6-dichloro-1-beta-D-benzimidazole-resistant short transcripts in vitro.

Détails

ID Serval
serval:BIB_D901532285A9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The HIV-1 inducer of short transcripts activates the synthesis of 5,6-dichloro-1-beta-D-benzimidazole-resistant short transcripts in vitro.
Périodique
Journal of Biological Chemistry
Auteur(s)
Pessler F., Hernandez N.
ISSN
0021-9258[print], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
02/1998
Volume
273
Numéro
9
Pages
5375-5384
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The HIV-1 inducer of short transcripts (IST) is an unusual promoter element that activates the synthesis of short transcripts from the HIV-1 promoter as well as from heterologous promoters. While the DNA sequences constituting IST have been characterized in some detail, little is known about the biochemical mechanisms underlying IST activity. Here, we describe a cell-free transcription assay that faithfully reproduces the synthesis of IST-dependent HIV-1 short transcripts. As in vivo, formation of these short transcripts requires a functional IST element and is repressed in the presence of the viral trans-activator Tat. Short transcript and full-length transcript synthesis respond differently to variations in several reaction parameters, suggesting that the short and full-length transcripts are synthesized by transcription complexes with distinct biochemical properties. In particular, short transcript synthesis is resistant to the action of 5,6-dichloro-1-beta-D-benzimidazole, an inhibitor of transcript elongation. Formation of transcription complexes directed by the IST element may, therefore, not require the activity of a factor inhibited by 5, 6-dichloro-1-beta-D-benzimidazole, such as the TFIIH-associated or pTEFb kinases.
Mots-clé
Base Sequence, Benzimidazoles/pharmacology, Cell-Free System, DNA, Viral/genetics, Drug Resistance, Microbial/genetics, HIV-1/genetics, Molecular Sequence Data, Mutation, Promoter Regions, Genetic, RNA Polymerase II/metabolism, RNA, Viral/biosynthesis, Transcription, Genetic/drug effects
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 16:58
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