First detection of Waddlia chondrophila in Africa using SYBR Green real-time PCR on veterinary samples.

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Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_D8DEC9B9ABB5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
First detection of Waddlia chondrophila in Africa using SYBR Green real-time PCR on veterinary samples.
Périodique
Veterinary Microbiology
Auteur(s)
Barkallah M., Fendri I., Dhieb A., Gharbi Y., Greub G., Gdoura R.
ISSN
1873-2542 (Electronic)
ISSN-L
0378-1135
Statut éditorial
Publié
Date de publication
2013
Volume
164
Numéro
1-2
Pages
101-107
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Waddlia chondrophila is a strict intracellular microorganism belonging to the order Chlamydiales that has been isolated twice from aborted bovine fetuses, once in USA and once in Germany. This bacterium is now considered as an abortigenic agent in cattle. However, no information is available regarding the presence of this bacterium in Africa. Given the low sensitivity of cell culture to recover such an obligate intracellular bacterium, molecular-based diagnostic approaches are warranted. This report describes the development of a quantitative SYBR Green real-time PCR assay targeting the recA gene of W. chondrophila. Analytical sensitivity was 10 copies of control plasmid DNA per reaction. No cross-amplification was observed when testing pathogens that can cause abortion in cattle. The PCR exhibited a good intra-run and inter-run reproducibility. This real-time PCR was then applied to 150 vaginal swabs taken from Tunisian cows that have aborted. Twelve samples revealed to be Waddlia positive, suggesting a possible role of this bacterium in this setting. This new real-time PCR assay represents a diagnostic tool that may be used to further study the prevalence of Waddlia infection.
Pubmed
Web of science
Open Access
Oui
Création de la notice
31/05/2013 17:06
Dernière modification de la notice
09/05/2019 1:58
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