How to build phylogenetic species trees with OMA.

Details

Ressource 1Download: 35722083_BIB_D8D72D90D99B.pdf (2317.11 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_D8D72D90D99B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
How to build phylogenetic species trees with OMA.
Journal
F1000Research
Author(s)
Dylus D., Nevers Y., Altenhoff A.M., Gürtler A., Dessimoz C., Glover N.M.
ISSN
2046-1402 (Electronic)
ISSN-L
2046-1402
Publication state
Published
Issued date
2020
Peer-reviewed
Oui
Volume
9
Pages
511
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Knowledge of species phylogeny is critical to many fields of biology. In an era of genome data availability, the most common way to make a phylogenetic species tree is by using multiple protein-coding genes, conserved in multiple species. This methodology is composed of several steps: orthology inference, multiple sequence alignment and inference of the phylogeny with dedicated tools. This can be a difficult task, and orthology inference, in particular, is usually computationally intensive and error prone if done ad hoc. This tutorial provides protocols to make use of OMA Orthologous Groups, a set of genes all orthologous to each other, to infer a phylogenetic species tree. It is designed to be user-friendly and computationally inexpensive, by providing two options: (1) Using only precomputed groups with species available on the OMA Browser, or (2) Computing orthologs using OMA Standalone for additional species, with the option of using precomputed orthology relations for those present in OMA. A protocol for downstream analyses is provided as well, including creating a supermatrix, tree inference, and visualization. All protocols use publicly available software, and we provide scripts and code snippets to facilitate data handling. The protocols are accompanied with practical examples.
Keywords
OMA, Orthologous Matrix, phylogenetics, phylogenomics, species tree
Pubmed
Open Access
Yes
Create date
04/04/2023 16:08
Last modification date
23/01/2024 8:35
Usage data