Article: article from journal or magazin.
Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas.
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
We investigated the expression of tight junction proteins in human lung squamous cell carcinomas and adenocarcinomas by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). We found a statistically significant correlation between diagnosis and positivity of tumors with either claudin (CLDN)-1 or CLDN-5. Squamous cell carcinomas and basal cells of bronchial epithelium were positive for CLDN-1 and negative for CLDN-5, whereas adenocarcinomas, normal cylindrical cells and pneumocytes were positive for CLDN-5 and negative for CLDN-1, suggesting different pathways in tumor development and progression. CLDN-4 and ZO-1 staining were detected in both types of tumors, whereas cingulin (CGN) was not detected in squamous cell carcinomas. Quantitative RT-PCR was used to evaluate changes in transcript levels for a large panel of tight junction proteins. In squamous cell carcinomas, we observed statistically significant decreases in the mRNA levels of JAM-1, occludin, CLDN-3, CLDN-4, CLDN-7, CGN, ZO-2 and ZO-3, and an increase in CLDN-1 mRNA. In adenocarcinomas, when transcript levels were compared with bronchial cells, we observed statistically significant decreases in the mRNA levels of CLDN-1, CLDN-3, CLDN-4, CLDN-7, ZO-2 and ZO-3. These results indicate that characterization of tight junction protein expression in human lung tumors can be an additional diagnostic tool and provide new insights on their histogenesis.
Adenocarcinoma/chemistry, Adenocarcinoma/diagnosis, Adult, Aged, Aged, 80 and over, Carcinoma, Squamous Cell/chemistry, Carcinoma, Squamous Cell/diagnosis, Claudin-1, Claudin-4, Claudin-5, Diagnosis, Differential, Female, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, Lung Neoplasms/chemistry, Lung Neoplasms/diagnosis, Male, Membrane Proteins/analysis, Membrane Proteins/genetics, Microfilament Proteins/analysis, Middle Aged, Neoplasm Staging, Phosphoproteins/analysis, RNA, Messenger/analysis, Reverse Transcriptase Polymerase Chain Reaction, Tight Junctions/chemistry, Tight Junctions/genetics, Tumor Markers, Biological/analysis, Tumor Markers, Biological/genetics, Zonula Occludens-1 Protein
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