Article: article from journal or magazin.
Cell refractive index tomography by digital holographic microscopy.
For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.
Algorithms, Holography/methods, Image Enhancement/methods, Image Interpretation, Computer-Assisted/methods, Imaging, Three-Dimensional/methods, Microscopy/methods, Pollen/ultrastructure, Refractometry/methods, Signal Processing, Computer-Assisted, Tomography, Optical/methods
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