Plasmodium berghei-infected primary hepatocytes process and present the circumsporozoite protein to specific CD8+ T cells in vitro

Details

Serval ID
serval:BIB_D6899D4AD196
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Plasmodium berghei-infected primary hepatocytes process and present the circumsporozoite protein to specific CD8+ T cells in vitro
Journal
Journal of Immunology
Author(s)
Bongfen  S. E., Torgler  R., Romero  J. F., Renia  L., Corradin  G.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
06/2007
Volume
178
Number
11
Pages
7054-63
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 1
Abstract
A substantial and protective response against malaria liver stages is directed against the circumsporozoite protein (CSP) and involves induction of CD8(+) T cells and production of IFN-gamma. CSP-derived peptides have been shown to be presented on the surface of infected hepatocytes in the context of MHC class I molecules. However, little is known about how the CSP and other sporozoite Ags are processed and presented to CD8(+) T cells. We investigated how primary hepatocytes from BALB/c mice process the CSP of Plasmodium berghei after live sporozoite infection and present CSP-derived peptides to specific H-2K(d)-restricted CD8(+) T cells in vitro. Using both wild-type and spect(-/-) P. berghei sporozoites, we show that both infected and traversed primary hepatocytes process and present the CSP. The processing and presentation pathway was found to involve the proteasome, Ag transport through a postendoplasmic reticulum compartment, and aspartic proteases. Thus, it can be hypothesized that infected hepatocytes can contribute in vivo to the elicitation and expansion of a T cell response.
Keywords
Animals Antigen Presentation/*immunology Aspartic Endopeptidases/physiology CD8-Positive T-Lymphocytes/*immunology/*metabolism/parasitology Cell Line, Tumor Epitopes, T-Lymphocyte/immunology/*metabolism Hepatocytes/enzymology/*immunology/*metabolism/parasitology Humans Mice Mice, Inbred BALB C Mice, Inbred C57BL Plasmodium berghei/*immunology Plasmodium falciparum/immunology Proteasome Endopeptidase Complex/physiology Protozoan Proteins/*immunology/metabolism
Pubmed
Web of science
Create date
24/01/2008 14:55
Last modification date
20/08/2019 15:56
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