Gene expression analysis in cartilage by in situ hybridization.

Détails

ID Serval
serval:BIB_D680CCF386EF
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Titre
Gene expression analysis in cartilage by in situ hybridization.
Titre du livre
Cartilage and Osteoarthritis. Volume 1: Cellular and Molecular Tools.
Auteur(s)
Mallein-Gerin F., Gouttenoire J.
Editeur
Sabatini M, Pastoureau P and De Ceuninck F
ISSN
1543-1894 (Print)
Statut éditorial
Publié
Date de publication
2004
Volume
100
Pages
105-108
Edition
Humana Press, Totowa, NJ
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
In situ hybridization allows detection and localization of specific nucleic acid sequences directly within a cell or tissue. We present an in situ hybridization protocol using double-stranded DNA or single-stranded RNA probes labeled with [32P] to localize and visualize the temporal and spatial distribution of cartilage-characteristic mRNAs. Probes labeled with this high-energy isotope provide good resolution at the tissue level with relatively low background; as a result of the probes that can be obtained that have a higher specificity to emulsion activity, very short exposure times are required.
Mots-clé
Animals, Cartilage/chemistry, Cartilage/metabolism, Collagen Type II/genetics, Collagen Type X/genetics, DNA Probes/chemistry, Gene Expression/genetics, In Situ Hybridization/methods, Leg Bones/chemistry, Mice, RNA Probes/chemistry, RNA, Messenger/analysis
Pubmed
Création de la notice
01/10/2015 16:35
Dernière modification de la notice
21/08/2019 6:34
Données d'usage