Lung CD11c+ cells from mice deficient in Epstein-Barr virus-induced gene 3 (EBI-3) prevent airway hyper-responsiveness in experimental asthma

Details

Serval ID
serval:BIB_D5ACE750BB6C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Lung CD11c+ cells from mice deficient in Epstein-Barr virus-induced gene 3 (EBI-3) prevent airway hyper-responsiveness in experimental asthma
Journal
European Journal of Immunology
Author(s)
Hausding  M., Karwot  R., Scholtes  P., Lehr  H. A., Wegmann  M., Renz  H., Galle  P. R., Birkenbach  M., Neurath  M. F., Blumberg  R. S., Finotto  S.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
2007
Volume
37
Number
6
Pages
1663-1677
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
Epstein-Barr virus-induced gene (EBI)-3 codes for a soluble type 1 cytokine receptor homologous to the p40 subunit of IL-12 that is expressed by antigen-presenting cells following activation. Here, we analyzed the functional role of EBI-3 in a murine model of asthma associated with airway hyper-responsiveness (AHR) in ovalbumin-sensitized mice. Upon allergen challenge, EBI-3-/- mice showed less severe AHR, decreased numbers and degranulation of eosinophils and a significantly reduced number of VCAM-1+ cells in the lungs as compared to wild-type littermates. We thus analyzed lung CD11c+ cells before and after allergen challenge in these mice and found that before allergen challenge, lung CD11c+ cells isolated from EBI-3-/- mice express markers of a more plasmacytoid phenotype without releasing IFN-alpha as compared to those from wild-type littermates. Moreover, allergen challenge induced the development of myeloid CD11c+ cells in the lungs of EBI-3-/- mice, which released increased amounts of IL-10 and IL-12 while not expressing IFN-alpha. Finally, inhibition of EBI-3 expression in lung DC could prevent AHR in adoptive transfer studies by suppressing mediator release of effector cells into the airways. These results indicate a novel role for EBI-3 in controlling local immune responses in the lungs in experimental asthma
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2008 18:35
Last modification date
20/08/2019 15:55
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