Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m<sup>6</sup>A machinery component Wtap/Fl(2)d.

Détails

ID Serval
serval:BIB_D5A1EF799866
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m<sup>6</sup>A machinery component Wtap/Fl(2)d.
Périodique
Genes & development
Auteur(s)
Knuckles P., Lence T., Haussmann I.U., Jacob D., Kreim N., Carl S.H., Masiello I., Hares T., Villaseñor R., Hess D., Andrade-Navarro M.A., Biggiogera M., Helm M., Soller M., Bühler M., Roignant J.Y.
ISSN
1549-5477 (Electronic)
ISSN-L
0890-9369
Statut éditorial
Publié
Date de publication
01/03/2018
Peer-reviewed
Oui
Volume
32
Numéro
5-6
Pages
415-429
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
N <sup>6</sup> -methyladenosine (m <sup>6</sup> A) is the most abundant mRNA modification in eukaryotes, playing crucial roles in multiple biological processes. m <sup>6</sup> A is catalyzed by the activity of methyltransferase-like 3 (Mettl3), which depends on additional proteins whose precise functions remain poorly understood. Here we identified Zc3h13 (zinc finger CCCH domain-containing protein 13)/Flacc [Fl(2)d-associated complex component] as a novel interactor of m <sup>6</sup> A methyltransferase complex components in Drosophila and mice. Like other components of this complex, Flacc controls m <sup>6</sup> A levels and is involved in sex determination in Drosophila We demonstrate that Flacc promotes m <sup>6</sup> A deposition by bridging Fl(2)d to the mRNA-binding factor Nito. Altogether, our work advances the molecular understanding of conservation and regulation of the m <sup>6</sup> A machinery.
Mots-clé
Adenosine/metabolism, Animals, Carrier Proteins/metabolism, Cell Line, DNA-Binding Proteins/metabolism, Drosophila Proteins/metabolism, Drosophila melanogaster/enzymology, Drosophila melanogaster/physiology, Gene Expression Regulation, Developmental, Methylation, Methyltransferases/metabolism, Mice, Mouse Embryonic Stem Cells, Nuclear Proteins/metabolism, Protein Transport, RNA Precursors/genetics, RNA Splicing, RNA-Binding Proteins/metabolism, Sex Determination Processes/genetics, Flacc, RNA modifications, Zc3h13, m6A, methyltransferase complex, sex determination
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/10/2019 12:42
Dernière modification de la notice
29/10/2019 6:26
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