Rab3A and calmodulin regulate acrosomal exocytosis by mechanisms that do not require a direct interaction

Details

Serval ID
serval:BIB_D560B773F1D7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rab3A and calmodulin regulate acrosomal exocytosis by mechanisms that do not require a direct interaction
Journal
FEBS Letters
Author(s)
Yunes  R., Tomes  C., Michaut  M., De Blas  G., Rodriguez  F., Regazzi  R., Mayorga  L. S.
ISSN
0014-5793 (Print)
Publication state
Published
Issued date
08/2002
Volume
525
Number
1-3
Pages
126-30
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 14
Abstract
The interaction between Rab3A and calmodulin is necessary for the inhibitory effect of Rab3A in neuroendocrine cells. Contrastingly, Rab3A triggers the exocytosis known as acrosome reaction in permeabilized spermatozoa. Here we show that a Rab3A mutant that cannot bind calmodulin was fully capable of triggering acrosomal exocytosis. Additionally, calmodulin by itself abrogated the exocytosis triggered by Rab3A. The effect was observed with both the wild type protein and the calmodulin binding deficient mutant. Our results indicate that the inhibitory and stimulatory effects of Rab3A in different exocytic processes are mediated by different effectors.
Keywords
Acrosome Reaction/drug effects/*physiology Calcimycin/pharmacology Calmodulin/antagonists & inhibitors/*metabolism/pharmacology Cell Membrane Permeability Chlorpromazine/pharmacology Dopamine Antagonists/pharmacology Enzyme Inhibitors/pharmacology Exocytosis/drug effects/*physiology Fluorescent Dyes Humans Ionophores/pharmacology Male Mutation Progesterone/pharmacology Protein Binding/physiology Spermatozoa/cytology/drug effects/metabolism rab3A GTP-Binding Protein/genetics/*metabolism/pharmacology
Pubmed
Web of science
Create date
24/01/2008 14:30
Last modification date
20/08/2019 15:55
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