Article: article from journal or magazin.
Expression of CD94/NKG2-A on human T lymphocytes is induced by IL-12: implications for adoptive immunotherapy.
Journal of Immunology
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
NK cell receptors (NKRs) are expressed on a subset of human T cells, predominantly CD8(+), within which they can modulate TCR-mediated functions. In an attempt to identify the mechanisms leading to NKR expression, we analyzed the capacity of IL-12 to modulate the expression by T cells of the components of the CD94/NKG2-A inhibitory receptor, a member of the C-type lectin-like family of NKR. We show that IL-12 induces the expression of NKG2-A and/or CD94 by CD8(+) T cells in culture, and that this induction was mediated neither by IFN-gamma nor by IL-15. We also show, using the redirected killing assay, that IL-12-induced expression of both CD94 and NKG2-A led to the acquisition by T cells of a functional inhibitory receptor. Expression of the CD94/NKG2-A inhibitory receptor was also induced by IL-12 during T cell Ag stimulation so that in the presence of this cytokine a high proportion of melanoma-reactive CTL induced from PBL by melanoma peptide stimulation expressed this receptor. This study emphasizes the implication of IL-12 in the modulation of immune responses through NKR induction.
Antigens, CD/biosynthesis, CD8-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/metabolism, Clone Cells, Epitopes, T-Lymphocyte/immunology, Humans, Immunotherapy, Adoptive/methods, Interferon-gamma/physiology, Interleukin-12/physiology, Interleukin-15/physiology, Killer Cells, Natural/immunology, Killer Cells, Natural/metabolism, Lectins, C-Type, Lymphocyte Activation/immunology, Melanoma/immunology, Membrane Glycoproteins/biosynthesis, NK Cell Lectin-Like Receptor Subfamily C, NK Cell Lectin-Like Receptor Subfamily D, Neoplasm Proteins/pharmacology, Peptides/pharmacology, Receptors, Immunologic/biosynthesis, Receptors, Natural Killer Cell, T-Lymphocyte Subsets/immunology, T-Lymphocyte Subsets/metabolism, Tumor Cells, Cultured
Web of science
Last modification date