Transport of salicylate in proximal tubule (S2 segment) isolated from rabbit kidney

Détails

ID Serval
serval:BIB_D340E14F88E8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transport of salicylate in proximal tubule (S2 segment) isolated from rabbit kidney
Périodique
American Journal of Physiology
Auteur(s)
Schild  L., Roch-Ramel  F.
ISSN
0363-6127
Statut éditorial
Publié
Date de publication
04/1988
Volume
254
Numéro
4 Pt 2
Pages
F554-61
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
The secretory and reabsorptive transport of salicylate was studied in the isolated and perfused rabbit proximal tubule (S2 segment). Salicylate secretion (Jb----lsal) fulfilled the criteria for a carrier-mediated transport system: Jb----lsal was saturable, was reversibly inhibited by probenecid, and occurred against a concentration gradient. The Km and Vmax for this secretory transport were 80 microM and 3,200 fmol.min-1.mm-1, respectively. At luminal pH of 7.4 and 6.6, salicylate reabsorption (Jl----bsal) was low (100 fmol.min-1.mm-1). Jl----bsal was stimulated by increasing the bath PCO2 or by removing basolateral HCO3-; Jl----bsal was inhibited by ethoxyzolamide and by SITS in the bath. Our results indicate that salicylate reabsorption depends on H+ secretion, consistent with reabsorption by simple nonionic diffusion. When salicylate was present in the lumen only, Jl----bsal increased after inhibition of the secretory transport by adding ouabain or probenecid in the bath or by lowering the bath temperature. These results are compatible with luminal recycling of salicylate, and suggest the presence of a mediated secretory transporter located at the luminal membrane.
Mots-clé
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/analogs & derivatives/pharmacology Animals Carbon Radioisotopes Female Kidney Tubules, Proximal/drug effects/*metabolism Kinetics Male Ouabain/pharmacology Probenecid/pharmacology Rabbits Reference Values Salicylic Acid Salicylic Acids/*metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 13:55
Dernière modification de la notice
20/08/2019 16:53
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