Nasal immunization of mice with virus-like particles protects offspring against rotavirus diarrhea.

Détails

ID Serval
serval:BIB_D1D101F72124
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Nasal immunization of mice with virus-like particles protects offspring against rotavirus diarrhea.
Périodique
Journal of virology
Auteur(s)
Coste A., Sirard J.C., Johansen K., Cohen J., Kraehenbuhl J.P.
ISSN
0022-538X
Statut éditorial
Publié
Date de publication
2000
Peer-reviewed
Oui
Volume
74
Numéro
19
Pages
8966-71
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Rotavirus is the major cause of diarrhea among young infants in both humans and animals. Immune protection of newborns by vaccination is difficult to achieve since there is not enough time to mount an immune response before exposure to the virus. We have designed a vaccination strategy mediating transfer of neutralizing antibodies from the mother to the offspring during pregnancy and/or lactation. Adult female mice were nasally immunized with virus-like particles (VLPs) made of viral proteins VP2 and 6 (VLP2/6) or VP 2, 6, and 7 (VLP2/6/7) derived from the RF rotavirus strain in the presence or absence of cholera toxin. Both vaccines elicited serum and milk antibodies against the respective VPs. Four days after parturition, suckling pups were challenged orally with RF rotavirus. Pups from mothers immunized with VLP2/6/7 but not VLP2/6 were protected against rotavirus diarrhea, indicating that VP7 plays a key role in protection. Protection was mediated by milk rather than serum antibodies, and mucosal adjuvants were not required. In conclusion, VLPs containing VP7 administered nasally to mothers represent a promising vaccine candidate for the protection of suckling newborns against rotavirus-induced diarrhea, even in the absence of a mucosal adjuvant.
Mots-clé
Administration, Intranasal, Animals, Female, Immunity, Maternally-Acquired, Immunization, Mice, Mice, Inbred BALB C, Pregnancy, Rotavirus, Rotavirus Infections, Viral Proteins, Virion
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
09/05/2019 1:37
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