Occurrence of bacteria belonging to the order <i>Chlamydiales</i> was investigated for the first time in common toad ( <i>Bufo bufo</i> ) tadpole populations collected from 41 ponds in the Geneva metropolitan area, Switzerland. A <i>Chlamydiales</i> -specific real-time PCR was used to detect and amplify the <i>Chlamydiales</i> 16S ribosomal RNA-encoding gene from the tails of 375 tadpoles. We found the studied amphibian populations to host <i>Chlamydia</i> -like organisms (CLOs) attributable to the genera <i>Similichlamydia, Neochlamydia, Protochlamydia</i> and <i>Parachlamydia</i> (all belonging to the family <i>Parachlamydiaceae</i> ), <i>Simkania</i> (family <i>Simkaniaceae</i> ) and <i>Estrella</i> (family <i>Criblamydiaceae</i> ); additionally, DNA from the genus <i>Thermoanaerobacter</i> (family <i>Thermoanaerobacteriaceae</i> ) was detected. Global autocorrelation analysis did not reveal a spatial structure in the observed CLOs occurrence rates, and association tests involving land cover characteristics did not evidence any clear effect on CLOs occurrence rates in <i>B. bufo.</i> Although preliminary, these results suggest a random and ubiquitous distribution of CLOs in the environment, which would support the biogeographical expectation 'everything is everywhere' for the concerned microorganisms.