Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating.

Détails

Ressource 1Télécharger: 1467.full.pdf (3046.13 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_CFEC44C3FF8F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating.
Périodique
The Journal of cell biology
Auteur(s)
Merlini L., Khalili B., Dudin O., Michon L., Vincenzetti V., Martin S.G.
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Statut éditorial
Publié
Date de publication
02/04/2018
Peer-reviewed
Oui
Volume
217
Numéro
4
Pages
1467-1483
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Video-Audio Media
Publication Status: ppublish
Résumé
In the fission yeast Schizosaccharomyces pombe, pheromone signaling engages a signaling pathway composed of a G protein-coupled receptor, Ras, and a mitogen-activated protein kinase (MAPK) cascade that triggers sexual differentiation and gamete fusion. Cell-cell fusion requires local cell wall digestion, which relies on an initially dynamic actin fusion focus that becomes stabilized upon local enrichment of the signaling cascade on the structure. We constructed a live-reporter of active Ras1 (Ras1-guanosine triphosphate [GTP]) that shows Ras activity at polarity sites peaking on the fusion structure before fusion. Remarkably, constitutive Ras1 activation promoted fusion focus stabilization and fusion attempts irrespective of cell pairing, leading to cell lysis. Ras1 activity was restricted by the guanosine triphosphatase-activating protein Gap1, which was itself recruited to sites of Ras1-GTP and was essential to block untimely fusion attempts. We propose that negative feedback control of Ras activity restrains the MAPK signal and couples fusion with cell-cell engagement.
Mots-clé
Cell Polarity, Enzyme Activation, Feedback, Physiological, GTPase-Activating Proteins/genetics, GTPase-Activating Proteins/metabolism, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Kinetics, Mitogen-Activated Protein Kinases/metabolism, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/growth & development, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Schizosaccharomyces/enzymology, Schizosaccharomyces/genetics, Schizosaccharomyces/growth & development, Schizosaccharomyces pombe Proteins/genetics, Schizosaccharomyces pombe Proteins/metabolism, Signal Transduction, ras Proteins/genetics, ras Proteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/03/2018 21:26
Dernière modification de la notice
09/05/2019 1:31
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