Article: article from journal or magazin.
A combined array-based comparative genomic hybridization and functional library screening approach identifies mir-30d as an oncomir in cancer.
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Oncomirs are microRNAs (miRNA) that acts as oncogenes or tumor suppressor genes. Efficient identification of oncomirs remains a challenge. Here we report a novel, clinically guided genetic screening approach for the identification of oncomirs, identifying mir-30d through this strategy. mir-30d regulates tumor cell proliferation, apoptosis, senescence, and migration. The chromosomal locus harboring mir-30d was amplified in more than 30% of multiple types of human solid tumors (n = 1,283). Importantly, higher levels of mir-30d expression were associated significantly with poor clinical outcomes in ovarian cancer patients (n = 330, P = 0.0016). Mechanistic investigations suggested that mir-30d regulates a large number of cancer-associated genes, including the apoptotic caspase CASP3. The guided genetic screening approach validated by this study offers a powerful tool to identify oncomirs that may have utility as biomarkers or targets for drug development.
Animals, Apoptosis/genetics, Cell Proliferation, Chromosome Mapping, Comparative Genomic Hybridization, Female, Humans, In Situ Hybridization, Mice, MicroRNAs/genetics, Ovarian Neoplasms/genetics, Ovarian Neoplasms/pathology, Polymorphism, Single Nucleotide, Real-Time Polymerase Chain Reaction
Web of science
Last modification date