Common clonal origin of central and resident memory T cells following skin immunization

Details

Serval ID
serval:BIB_CF06D4FDBEF8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Common clonal origin of central and resident memory T cells following skin immunization
Journal
Nature Medicine
Author(s)
Gaide Olivier, Emerson Ryan O, Jiang Xiaodong, Gulati Nicholas, Nizza Suzanne, Desmarais Cindy, Robins Harlan, Krueger James G, Clark Rachael A, Kupper Thomas S
ISSN
1078-8956
1546-170X
Publication state
Published
Issued date
11/05/2015
Volume
21
Number
6
Pages
647-653
Language
english
Abstract
Central memory T (TCM) cells in lymph nodes (LNs) and resident memory T (TRM) cells in peripheral tissues have distinct roles in protective immunity. Both are generated after primary infections, but their clonal origins have been unclear. To address this question, we immunized mice through the skin with a protein antigen, a chemical hapten, or a non-replicating poxvirus. We then analyzed antigen-activated T cells from different tissues using high-throughput sequencing (HTS) of the gene encoding the T cell receptor (TCR) β-chain (Trb, also known as Tcrb) using CDR3 sequences to simultaneously track thousands of unique T cells. For every abundant TRM cell clone generated in the skin, an abundant TCM cell clone bearing the identical TCR was present in the LNs. Thus, antigen-reactive skin TRM and LN TCM cell clones were derived from a common naive T cell precursor after skin immunization, generating overlapping TCR repertoires. Although they bore the same TCR, TRM cells mediated rapid contact hypersensitivity responses, whereas TCM cells mediated delayed and attenuated responses. Studies in human subjects confirmed the generation of skin TRM cells in allergic contact dermatitis. Thus, immunization through skin simultaneously generates skin TRM and LN TCM cells in similar numbers from the same naive T cells.
Keywords
General Biochemistry, Genetics and Molecular Biology, General Medicine
Pubmed
Web of science
Create date
02/11/2017 10:55
Last modification date
20/08/2019 15:49
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