Bone marrow mesenchymal stem cells stabilize already-formed aortic aneurysms more efficiently than vascular smooth muscle cells in a rat model.

Details

Serval ID
serval:BIB_CEEF11558792
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Bone marrow mesenchymal stem cells stabilize already-formed aortic aneurysms more efficiently than vascular smooth muscle cells in a rat model.
Journal
European Journal of Vascular and Endovascular Surgery
Author(s)
Schneider F., Saucy F., de Blic R., Dai J., Mohand F., Rouard H., Ricco J.B., Becquemin J.P., Gervais M., Allaire E.
ISSN
1532-2165 (Electronic)
ISSN-L
1078-5884
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
45
Number
6
Pages
666-672
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
PURPOSE: Abdominal aortic aneurysms (AAAs) expand because of aortic wall destruction. Enrichment in Vascular Smooth Muscle Cells (VSMCs) stabilizes expanding AAAs in rats. Mesenchymal Stem Cells (MSCs) can differentiate into VSMCs. We have tested the hypothesis that bone marrow-derived MSCs (BM-MSCs) stabilizes AAAs in a rat model.
MATERIAL AND METHODS: Rat Fischer 344 BM-MSCs were isolated by plastic adhesion and seeded endovascularly in experimental AAAs using xenograft obtained from guinea pig. Culture medium without cells was used as control group. The main criteria was the variation of the aortic diameter at one week and four weeks. We evaluated the impact of cells seeding on inflammatory response by immunohistochemistry combined with RT-PCR on MMP9 and TIMP1 at one week. We evaluated the healing process by immunohistochemistry at 4 weeks.
RESULTS: The endovascular seeding of BM-MSCs decreased AAA diameter expansion more powerfully than VSMCs or culture medium infusion (6.5% ± 9.7, 25.5% ± 17.2 and 53.4% ± 14.4; p = .007, respectively). This result was sustained at 4 weeks. BM-MSCs decreased expression of MMP-9 and infiltration by macrophages (4.7 ± 2.3 vs. 14.6 ± 6.4 mm(2) respectively; p = .015), increased Tissue Inhibitor Metallo Proteinase-1 (TIMP-1), compared to culture medium infusion. BM-MSCs induced formation of a neo-aortic tissue rich in SM-alpha active positive cells (22.2 ± 2.7 vs. 115.6 ± 30.4 cells/surface units, p = .007) surrounded by a dense collagen and elastin network covered by luminal endothelial cells.
CONCLUSIONS: We have shown in this rat model of AAA that BM-MSCs exert a specialized function in arterial regeneration that transcends that of mature mesenchymal cells. Our observation identifies a population of cells easy to isolate and to expand for therapeutic interventions based on catheter-driven cell therapy.
Keywords
Animals, Aorta, Abdominal/metabolism, Aorta, Abdominal/pathology, Aortic Aneurysm, Abdominal/genetics, Aortic Aneurysm, Abdominal/metabolism, Bone Marrow Transplantation, Cell Differentiation, Cells, Cultured, Disease Models, Animal, Guinea Pigs, Immunohistochemistry, Male, Matrix Metalloproteinase 9/genetics, Matrix Metalloproteinase 9/metabolism, Mesenchymal Stem Cell Transplantation, Muscle, Smooth, Vascular/metabolism, Muscle, Smooth, Vascular/pathology, Myocytes, Smooth Muscle/metabolism, Myocytes, Smooth Muscle/pathology, Rats, Rats, Inbred F344, Regeneration, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Tissue Inhibitor of Metalloproteinase-1/genetics, Tissue Inhibitor of Metalloproteinase-1/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
04/08/2013 8:32
Last modification date
20/08/2019 15:49
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