Comparison between Stromal Vascular Fraction and Adipose Mesenchymal Stem Cells in Remodeling Hypertrophic Scars.

Détails

Ressource 1Télécharger: BIB_CE5C365DB6C3.P001.pdf (8267.26 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_CE5C365DB6C3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparison between Stromal Vascular Fraction and Adipose Mesenchymal Stem Cells in Remodeling Hypertrophic Scars.
Périodique
PloS one
Auteur(s)
Domergue S., Bony C., Maumus M., Toupet K., Frouin E., Rigau V., Vozenin M.C., Magalon G., Jorgensen C., Noël D.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
11
Numéro
5
Pages
e0156161
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Hypertrophic scars (HTS) are characterized by excessive amount of collagen deposition and principally occur following burn injuries or surgeries. In absence of effective treatments, the use of mesenchymal stem/stromal cells, which have been shown to attenuate fibrosis in various applications, seems of interest. The objectives of the present study were therefore to evaluate the effect of human adipose tissue-derived mesenchymal stem cells (hASC) on a pre-existing HTS in a humanized skin graft model in Nude mice and to compare the efficacy of hASCs versus stromal vascular fraction (SVF). We found that injection of SVF or hASCs resulted in an attenuation of HTS as noticed after clinical evaluation of skin thickness, which was associated with lower total collagen contents in the skins of treated mice and a reduced dermis thickness after histological analysis. Although both SVF and hASCs were able to significantly reduce the clinical and histological parameters of HTS, hASCs appeared to be more efficient than SVF. The therapeutic effect of hASCs was attributed to higher expression of TGFβ3 and HGF, which are important anti-fibrotic mediators, and to higher levels of MMP-2 and MMP-2/TIMP-2 ratio, which reflect the remodelling activity responsible for fibrosis resorption. These results demonstrated the therapeutic potential of hASCs for clinical applications of hypertrophic scarring.

Mots-clé
Adipose Tissue/cytology, Animals, Cells, Cultured, Cicatrix, Hypertrophic/prevention & control, Humans, Mesenchymal Stem Cell Transplantation, Mesenchymal Stromal Cells/cytology, Mice, Mice, Nude, Stromal Cells/cytology, Wound Healing/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/06/2016 16:20
Dernière modification de la notice
20/08/2019 15:48
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