Release of vancomycin and teicoplanin from a plasticized and resorbable gelatin sponge: in vitro investigation of a new antibiotic delivery system with glycopeptides.

Details

Serval ID
serval:BIB_CDD40723FF9F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Release of vancomycin and teicoplanin from a plasticized and resorbable gelatin sponge: in vitro investigation of a new antibiotic delivery system with glycopeptides.
Journal
Infection
Author(s)
Drognitz O., Thorn D., Krüger T., Gatermann S.G., Iven H., Bruch H.P., Muhl E.
ISSN
0300-8126 (Print)
ISSN-L
0300-8126
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
34
Number
1
Pages
29-34
Language
english
Notes
Publication types: Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
BACKGROUND: The aim of this study was to evaluate the efficacy of sustained release of vancomycin and teicoplanin from a resorbable gelatin glycerol sponge, in order to establish a new delivery system for local anti-infective therapy.
MATERIALS AND METHODS: 60 plasticized glycerol gelatin sponges containing either 10 or 20% gelatin (w/v) were incubated in vancomycin or teicoplanin solution at 20 degrees C for either 1 or 24 h. In vitro release properties of the sponges were investigated over a period of 1 week by determining the levels of vancomycin and teicoplanin eluted in plasma using fluorescent polarization immunoassay. The rate constant and the half-life for the antibiotic release of each group were calculated by linear regression assuming first order kinetics.
RESULTS: Presoaking for 24 h was associated with a significant increase in the total antibiotic release in all groups opposed to 1 h of incubation, except for the 10% sponges presoaked in teicoplanin. Doubling the gelatin content of the sponges from 10 to 20% significantly increased the total release of antibiotic load only in teicoplanin-containing sponges after 24 h incubation. In all corresponding groups investigated, release of vancomycin was more prolonged compared to teicoplanin, which allowed a gradual release beyond 5 days. The half-life (h +/- SEM) of both types of vancomycin-containing sponges was significantly prolonged by 24 h incubation in comparison to 1 h incubation (29.1 +/- 5.9 vs 5.9 +/- 1.0; p < 0.001, 30.0 +/- 2.1 vs 11.1 +/- 1.9; p < 0.001). However, neither doubling the gelatin content of the sponges nor a prolonged incubation was associated with a significantly prolonged delivery of teicoplanin.
CONCLUSION: This study demonstrated a better diffusion-controlled release of vancomycin-impregnated glycerol gelatin sponges compared to those pretreated with teicoplanin. The plasticized glycerol gelatin sponge may be a promising carrier for the application of vancomycin to infected wounds for local anti-infective therapy.
Keywords
Anti-Bacterial Agents/administration & dosage, Anti-Bacterial Agents/pharmacokinetics, Delayed-Action Preparations, Drug Delivery Systems/methods, Gelatin/chemistry, Glycopeptides/administration & dosage, Glycopeptides/pharmacokinetics, Half-Life, Plastics/chemistry, Teicoplanin/administration & dosage, Teicoplanin/pharmacokinetics, Vancomycin/administration & dosage, Vancomycin/pharmacokinetics
Pubmed
Web of science
Create date
28/02/2008 13:44
Last modification date
20/08/2019 16:48
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