Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets.

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Serval ID
serval:BIB_CD67A5ADF46E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets.
Journal
Journal of Translational Medicine
Author(s)
Salaun B., Yamamoto T., Badran B., Tsunetsugu-Yokota Y., Roux A., Baitsch L., Rouas R., Fayyad-Kazan H., Baumgaertner P., Devevre E., Ramesh A., Braun M., Speiser D., Autran B., Martiat P., Appay V., Romero P.
ISSN
1479-5876 (Electronic)
ISSN-L
1479-5876
Publication state
Published
Issued date
2011
Volume
9
Pages
44
Language
english
Abstract
BACKGROUND: The differentiation of CD8+ T lymphocytes following priming of naïve cells is central in the establishment of the adaptive immune response. Yet, the molecular events underlying this process are not fully understood. MicroRNAs have been recently shown to play a key role in the regulation of haematopoiesis in mouse, but their implication in peripheral lymphocyte differentiation in humans remains largely unknown.
METHODS: In order to explore the potential implication of microRNAs in CD8+ T cell differentiation in humans, microRNA expression profiles were analysed using microarrays and quantitative PCR in several human CD8+ T cell subsets defining the major steps of the T cell differentiation pathway.
RESULTS: We found expression of a limited set of microRNAs, including the miR-17~92 cluster. Moreover, we reveal the existence of differentiation-associated regulation of specific microRNAs. When compared to naive cells, miR-21 and miR-155 were indeed found upregulated upon differentiation to effector cells, while expression of the miR-17~92 cluster tended to concomitantly decrease.
CONCLUSIONS: This study establishes for the first time in a large panel of individuals the existence of differentiation associated regulation of microRNA expression in human CD8+ T lymphocytes in vivo, which is likely to impact on specific cellular functions.
Keywords
CD8-Positive T-Lymphocytes/metabolism, Cell Differentiation, Gene Expression Regulation, Humans, MicroRNAs/genetics, T-Lymphocyte Subsets
Pubmed
Web of science
Open Access
Yes
Create date
13/01/2012 15:45
Last modification date
20/08/2019 15:48
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