Production of IL 2 and IFN-gamma by T cells from malaria patients in response to Plasmodium falciparum or erythrocyte antigens in vitro

Details

Serval ID
serval:BIB_CD42AA3CE42C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Production of IL 2 and IFN-gamma by T cells from malaria patients in response to Plasmodium falciparum or erythrocyte antigens in vitro
Journal
Journal of Immunology
Author(s)
Troye-Blomberg  M., Andersson  G., Stoczkowska  M., Shabo  R., Romero  P., Patarroyo  M. E., Wigzell  H., Perlmann  P.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
11/1985
Volume
135
Number
5
Pages
3498-504
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
T cells from patients acutely infected with malaria exhibit a disease-related stimulation of DNA synthesis in response to Plasmodium falciparum antigen in vitro. This response is weak and short-lived, suggestive of induction of suppressor mechanisms. Exogenous T cell growth factor (IL 2) that was added to antigen-stimulated T cell cultures enhanced proliferation in antigen-responsive cultures, indicating that the lymphocytes expressed IL 2 receptors. In contrast, the addition of IL 2 to cultures that did not respond to antigen had no effect. Antigen-responsive cultures contained endogenous IL 2 as well, and the antigen-induced lymphocyte proliferation was correlated with IL 2 production. However, the results suggested that IL 2 production by the patients' T cells was insufficient or actively shut off, and that this was responsible for the premature cessation of their DNA synthesis. Supernatants from 60% of the T cell cultures treated with malaria antigen and from 30% treated with RBC ghost antigen contained interferon-gamma (IFN-gamma), as determined by a cytopathic effect inhibition assay combined with acid treatment and antibody neutralization or by an IFN-gamma-specific ELISA. There was no obvious correlation between antigen-induced lymphocyte proliferation and the presence of IFN-gamma in the culture supernatants. A high IFN-gamma activity was also seen in antigen-treated cultures from P. falciparum-immune donors living in highly endemic malaria areas. In contrast, no IFN-gamma was found in supernatants of antigen-treated T cells from healthy donors or patients with Plasmodium vivax malaria. Thus, the IFN-gamma activity of these cultures appears to reflect the presence of antigen-reactive T cells and may be useful as a sensitive indicator of cellular immunity in P. falciparum malaria.
Keywords
ABO Blood-Group System/*immunology Adult Animals Antigens, Protozoan/*immunology Humans Interferon Type II/analysis/*biosynthesis Interleukin-2/*biosynthesis/physiology Lymphocyte Activation Malaria/*immunology Mice Plasmodium falciparum/immunology T-Lymphocytes/immunology/*metabolism
Pubmed
Web of science
Create date
28/01/2008 11:28
Last modification date
20/08/2019 15:47
Usage data