A human monoclonal antiphospholipid antibody that is representative of serum antibodies and is germline encoded

Détails

ID Serval
serval:BIB_CC31E445F1E1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A human monoclonal antiphospholipid antibody that is representative of serum antibodies and is germline encoded
Périodique
Arthritis and Rheumatism
Auteur(s)
Harmer  I. J., Loizou  S., Thompson  K. M., So  A. K., Walport  M. J., Mackworth-Young  C.
ISSN
0004-3591 (Print)
Statut éditorial
Publié
Date de publication
08/1995
Volume
38
Numéro
8
Pages
1068-76
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Résumé
OBJECTIVE. To investigate the origins of antiphospholipid antibodies associated with thrombosis and other disorders that are found in patients with systemic lupus erythematosus and primary antiphospholipid syndrome (APS). METHODS. Characterization, idiotypic study, and nucleotide sequencing of a human monoclonal antiphospholipid antibody generated from a patient with primary APS. Identification of the germline genes from which the antibody is derived. RESULTS. A human monoclonal antibody, BH1, was generated. This antibody has ligand-binding properties that closely resemble those of the serum antiphospholipid antibodies found in our patient and in other individuals with APS: it recognizes negatively charged phospholipids, and has lupus anticoagulant activity; it does not bind to neutral phospholipids, or to single-stranded or double-stranded DNA. The relevance of BH1 to the patient's serum antibodies is supported by our idiotypic studies. BH1 is encoded by a new germline VH gene, and by a lambda light chain gene that displays > 99% homology with the V lambda III.1 germline gene. CONCLUSION. Serum antiphospholipid antibodies associated with thrombosis may be encoded by either germline or only slightly mutated variable-region genes.
Mots-clé
Amino Acid Sequence Animals Antibodies, Antiphospholipid/genetics/*immunology Antibodies, Monoclonal/genetics/*immunology Antibody Specificity Antiphospholipid Syndrome/genetics/immunology Base Sequence DNA/immunology Enzyme-Linked Immunosorbent Assay Gene Expression/immunology Genes, Immunoglobulin/genetics/immunology Germ-Line Mutation/immunology Humans Immunoglobulin Idiotypes/immunology Mice Molecular Sequence Data Phospholipids/immunology/metabolism Protein Binding/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 9:38
Dernière modification de la notice
09/05/2019 1:20
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