Survival of embryonic cardiac myocytes transplanted into host rat soleus muscle

Details

Serval ID
serval:BIB_CC1F4B2C6E0B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Survival of embryonic cardiac myocytes transplanted into host rat soleus muscle
Journal
Journal of Muscle Research and Cell Motility
Author(s)
Connold  A. L., Frischknecht  R., Vrbova  G.
ISSN
0142-4319 (Print)
Publication state
Published
Issued date
10/1995
Peer-reviewed
Oui
Volume
16
Number
5
Pages
481-9
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
Small fragments of embryonic hearts were transplanted either alongside or into a skeletal muscle (soleus) of an adult host. The implanted tissue grew, and survived for at least 6 months after transplantation. The graft was well vascularized and established a network of blood vessels that communicated with the host's circulation. This communication appeared to be established by the proliferation of blood vessels from the graft into the host tissues. The grafted tissue was rhythmically active and the rate of these contractions was similar to that of adult rat hearts. The frequency of the spontaneous contractions could be modified by acetylcholine. Exposure to acetylcholine lead to a reversible slowing of the rate of beating. The presence of gap junctions in the transplanted tissue was revealed by visualizing connexin 43 with a specific antibody. During early periods after grafting the gap junctions were scattered within the graft but over time they became aligned into rows, to prepare for the formation of intercalated discs. Thus embryonic heart grafted into, or alongside skeletal muscle is able to acquire a considerable degree of differentiation.
Keywords
Acetylcholine/pharmacology Animals Cell Division/physiology Cell Movement/physiology *Cell Transplantation Embryo/cytology Gap Junctions/physiology Heart/embryology Immunohistochemistry Microscopy, Video Muscle Contraction/physiology Muscle, Skeletal Myocardium/*cytology Neovascularization, Physiologic Rats Rats, Inbred F344 *Transplantation, Heterotopic
Pubmed
Web of science
Create date
25/01/2008 8:34
Last modification date
20/08/2019 15:46
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