Membrane mu poly(A) signal and 3' flanking sequences function as a transcription terminator for immunoglobulin-encoding genes.

Détails

ID Serval
serval:BIB_CBEB3630DA8A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Membrane mu poly(A) signal and 3' flanking sequences function as a transcription terminator for immunoglobulin-encoding genes.
Périodique
Gene
Auteur(s)
Fasel N.J., Rousseaux M., Déglon N., Govan H.L., Law R., Bron C., Wall R.
ISSN
0378-1119 (Print)
ISSN-L
0378-1119
Statut éditorial
Publié
Date de publication
1992
Volume
122
Numéro
2
Pages
297-304
Langue
anglais
Résumé
Developmentally regulated mechanisms involving alternative RNA splicing and/or polyadenylation, as well as transcription termination, are implicated in controlling the levels of secreted mu (mu s), membrane mu (mu m) and delta immunoglobulin (Ig) heavy chain mRNAs during B cell differentiation (mu gene encodes the mu heavy chain). Using expression vectors constructed with genomic DNA segments composed of the mu m polyadenylation signal region, we analyzed poly(A) site utilization and termination of transcription in stably transfected myeloma cells and in murine fibroblast L cells. We found that the gene segment containing the mu m poly(A) signals, along with 536 bp of downstream flanking sequence, acted as a transcription terminator in both myeloma cells and L cell fibroblasts. Neither a 141-bp DNA fragment (which directed efficient polyadenylation at the mu m site), nor the 536-bp flanking nucleotide sequence alone, were sufficient to obtain a similar regulation. This shows that the mu m poly(A) region plays a central role in controlling developmentally regulated transcription termination by blocking downstream delta gene expression. Because this gene segment exhibited the same RNA processing and termination activities in fibroblasts, it appears that these processes are not tissue-specific.
Mots-clé
Alternative Splicing, Animals, Base Sequence, Blotting, Northern, Gene Expression Regulation, Genes, Immunoglobulin, Genetic Vectors, Humans, Immunoglobulin mu-Chains/genetics, L Cells (Cell Line), Mice, Molecular Sequence Data, Poly A, Terminator Regions, Genetic, Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
24/01/2008 16:02
Dernière modification de la notice
03/03/2018 21:27
Données d'usage