Fluorescent-based quantitative measurements of signal transduction in single cells

Détails

ID Serval
serval:BIB_CBA7FBEC6499
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Titre
Fluorescent-based quantitative measurements of signal transduction in single cells
Titre du livre
Design and Analysis of Biomolecular Circuits : engineering approaches to systems and synthetic biology
Auteur(s)
Pelet S., Peter M.
Editeur
Springer
Lieu d'édition
New York
ISBN
978-1-4419-6765-7
Statut éditorial
Publié
Date de publication
2011
Editeur scientifique
Koeppl H., Densmore D., Setti G., di Bernardo M.
Numéro de chapitre
17
Pages
369-393
Langue
anglais
Résumé
Budding yeast (Saccharomyces cerevisiae) has been widely used as a model system to study fundamental biological processes. Genetic and biochemical approaches have allowed in the last decades to uncover the key components involved in many signaling pathways. Generally, most techniques measure the average behavior of a population of cells, and thus miss important cell-to-cell variations. With the recent progress in fluorescent proteins, new avenues have been opened to quantitatively study the dynamics of signaling in single living cells. In this chapter, we describe several techniques based on fluorescence measurements to quantify the activation of biological pathways. Flow cytometry allows for rapid quantification of the total fluorescence of a large number of single cells. In contrast, microscopy offers a lower throughput but allows to follow with a high temporal resolution the localization of proteins at sub-cellular resolution. Finally, advanced functional imaging techniques such as FRET and FCS offer the possibility to directly visualize the formation of protein complexes or to quantify the activity of proteins in vivo. Together these techniques present powerful new approaches to study cellular signaling and will greatly increase our understanding of the regulation of signaling networks in budding yeast and beyond.
Mots-clé
Cellular signaling, Fluorescent proteins, Microscopy, Flow cytometry, FRET, FCS
Création de la notice
26/09/2012 11:39
Dernière modification de la notice
03/03/2018 21:26
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