False human cytomegalovirus IgG-positivity at prenatal screening

Details

Serval ID
serval:BIB_CB60C458FCA5
Type
Article: article from journal or magazin.
Collection
Publications
Title
False human cytomegalovirus IgG-positivity at prenatal screening
Journal
J Clin Virol
Author(s)
Furione M., Sarasini A., Arossa A., Fornara C., Lilleri D., Perez L., Parea M., Zavattoni M., Spinillo A., Marone P., Baldanti F.
ISSN
1386-6532
Publication state
Published
Issued date
07/2018
Volume
104
Pages
34-38
Language
english
Notes
1873-5967
Furione, Milena
Sarasini, Antonella
Arossa, Alessia
Fornara, Chiara
Lilleri, Daniele
Perez, Laurent
Parea, Maurizio
Zavattoni, Maurizio
Spinillo, Arsenio
Marone, Piero
Baldanti, Fausto
Journal Article
Research Support, Non-U.S. Gov't
Netherlands
J Clin Virol. 2018 Jul;104:34-38. doi: 10.1016/j.jcv.2018.04.009. Epub 2018 Apr 21.
Abstract
BACKGROUND: An incorrect definition of immune status to human cytomegalovirus (HCMV) can lead to incorrect management of pregnant women. OBJECTIVES: Aims of the study were: i) to describe 10 cases of unconfirmed HCMV IgG-seroconversion in pregnancy; ii) to develop a panel of confirmatory tests to define HCMV serostatus; iii) to investigate the frequency of false IgG-positive results in pregnant women screened with the LIAISON(®)CMVIgGII automated assay. STUDY DESIGN: Blood samples from 10 pregnant women referred for HCMV IgG-seroconversion were examined to confirm/exclude a primary infection. In addition, samples were tested for HCMV IgG by immunoblotting, neutralization assay, and ELISA against gB, gH/gL/pUL128L and gH/gL/gO recombinant glycoproteins. LIAISON(®)CMVIgGII results obtained on 1158 pregnant women were reviewed and samples with low IgG titers were further investigated. RESULTS: A primary infection was excluded in the 10 women referred for HCMV IgG seroconversion. None of them was confirmed to be IgG-seropositive. Of the 1158 women prenatally screened by LIAISON(®)CMVIgGII, 678 (59%) were IgG-positive and, of these, 40 (5.9%) showed low levels of IgG (14-50 U/mL). Thirty-three women with low IgG-positivity were further tested by confirmatory tests and 11 (33.3%) were found to be non reactive to HCMV. CONCLUSIONS: At least 1.6% (11/678) women who tested positive with LIAISON(®)CMVIgGII were found to be seronegative when tested with confirmatory tests. These women should be informed to reduce the risk of a primary HCMV infection. Furthermore, should a congenital infection occur in any of these women, a maternal non-primary infection could be erroneously diagnosed.
Keywords
Antibodies, Viral/*blood, Cytomegalovirus/*immunology, Cytomegalovirus Infections/*diagnosis, *False Positive Reactions, Female, Humans, Immunoglobulin G/*blood, Pregnancy, *Prenatal Diagnosis, Prevalence, Retrospective Studies, *Confirmatory tests, *Fully automatized assays, *Human cytomegalovirus, *Non-primary infection, *Pregnancy, *Prenatal screening
Create date
04/09/2020 20:03
Last modification date
07/09/2020 6:26
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