Article: article from journal or magazin.
In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts.
Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.
Animals, Cell Line, Tumor, Cell Movement/physiology, Collagen/metabolism, Extracellular Matrix/metabolism, Extracellular Matrix/pathology, Fibroblasts, Humans, Image Enhancement/methods, Integrin beta Chains/metabolism, Mice, Mice, Transgenic, Microscopy, Confocal/methods, Relaxin/pharmacology, Soft Tissue Neoplasms/metabolism, Soft Tissue Neoplasms/pathology, Stromal Cells/metabolism, Stromal Cells/pathology
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