Oligoclonal expansion of major histocompatibility complex class I-restricted cytolytic T lymphocytes during a primary immune response in vivo: direct monitoring by flow cytometry and polymerase chain reaction

Détails

ID Serval
serval:BIB_CAD587898C42
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Oligoclonal expansion of major histocompatibility complex class I-restricted cytolytic T lymphocytes during a primary immune response in vivo: direct monitoring by flow cytometry and polymerase chain reaction
Périodique
Journal of Experimental Medicine
Auteur(s)
MacDonald  H. R., Casanova  J. L., Maryanski  J. L., Cerottini  J. C.
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
05/1993
Volume
177
Numéro
5
Pages
1487-92
Notes
Journal Article --- Old month value: May 1
Résumé
Previous T cell receptor (TCR) sequence analysis of a panel of 23 H-2Kd restricted cytotoxic T lymphocyte (CTL) clones recognizing the decapeptide HLA-CW3 170-179 revealed a striking conservation of TCR structure, in that all clones examined used V beta 10 and J alpha pHDS58 segments. We show here that the primary response in vivo after intraperitoneal injection of DBA/2 mice with HLA-CW3 expressing transfectants of syngeneic P815 (H-2d) tumor cells is characterized by a dramatic expansion of CD8+ V beta 10+ CTL in the peritoneal cavity and draining (mesenteric) lymph node, as well as in peripheral blood. Additional analysis of TCR on HLA-CW3 immune populations by flow cytometry and polymerase chain reaction further indicates that the vast majority of responding CD8+ cells express restricted V alpha domains, a dominant J alpha segment (pHDS58), and a conserved CDR3 length for both alpha and beta chains. This novel system provides a unique opportunity to directly monitor an oligoclonal primary antigen specific immune response in vivo at the single cell level independently of functional assays.
Mots-clé
Animals Antigens, CD8 Cells, Cultured Clone Cells Female Flow Cytometry HLA-C Antigens/immunology Histocompatibility Antigens Class I/*immunology Mice Mice, Inbred DBA Peptide Fragments/immunology Polymerase Chain Reaction Receptors, Antigen, T-Cell, alpha-beta T-Lymphocytes, Cytotoxic/cytology/*immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:13
Dernière modification de la notice
09/05/2019 1:16
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