Article: article from journal or magazin.
DNA in human and stallion spermatozoa forms local hexagonal packing with twist and many defects.
Journal of Structural Biology
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
In human and other mammal sperm nuclei, DNA is packed in a highly condensed state, the structure of which remains unsolved. Cryoelectron microscopy of vitrified sections provides a first direct view of the local arrangement of the nucleoprotamine filament. DNA aligns in parallel in layers and its orientation rotates along a single-twist direction as in a cholesteric liquid crystal. The structure contains numerous defects, which introduce locally double-twist configurations. Destruction of the SS bonds with dithiotrehitol relaxes the twist and favors the extension of the hexagonal close packing of the filaments, though keeping constant their interfilament distance.
Animals, Chromatin/chemistry, Cryoelectron Microscopy, Crystallization, DNA/chemistry, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/drug effects, Dithiothreitol/pharmacology, Horses/physiology, Humans, Male, Models, Molecular, Molecular Conformation, Spermatozoa/chemistry
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