Identification of a new peptide recognized by autologous cytolytic T lymphocytes on a human melanoma

Détails

ID Serval
serval:BIB_CA9EFA214980
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Identification of a new peptide recognized by autologous cytolytic T lymphocytes on a human melanoma
Périodique
Cancer Immunity
Auteur(s)
Vigneron  N., Ooms  A., Morel  S., Degiovanni  G., Van Den Eynde  B. J.
ISSN
1424-9634 (Electronic)
Statut éditorial
Publié
Date de publication
07/2002
Volume
2
Pages
9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 19
Résumé
Melanoma line LG2-MEL expresses several antigens recognized by autologous CTLs. One of them consists of a peptide derived from tyrosinase and presented by HLA-B*3503. We have identified another antigen of LG2-MEL as a peptide presented by HLA-B*4403 and resulting from a point mutation in gene OS-9. This gene is expressed in various normal tissues. It is located on chromosome 12 in the vicinity of the CDK4 locus and is frequently co-amplified with CDK4 in human sarcomas. The mutation, a C-to-T transition, changes a proline residue into a leucine at position 446 of the OS-9 protein. Mutated transcripts were found in all the melanoma sublines of LG2-MEL. None of the 184 tumor samples collected from other cancer patients expressed the mutated transcript, indicating that this is a rare mutational event. Interestingly, some of the melanoma sublines of LG2-MEL have lost the wild-type allele of gene OS-9. Those sublines appear to grow faster in vitro than the sublines that retained the wild-type allele, suggesting that this loss of heterozygosity may favor tumor progression. The mutation we have identified in gene OS-9 might therefore participate in the oncogenic process by affecting the function of this potential tumor-suppressor gene.
Mots-clé
Amino Acid Sequence Animals Antigens, Neoplasm/chemistry/genetics/*immunology Base Sequence COS Cells Clone Cells Cytotoxicity Tests, Immunologic DNA, Complementary HLA-B Antigens/metabolism Humans Melanoma/genetics/*immunology Molecular Sequence Data Neoplasm Proteins/chemistry/genetics/*immunology Point Mutation T-Lymphocytes, Cytotoxic/*immunology Tumor Cells, Cultured
Pubmed
Création de la notice
28/01/2008 10:36
Dernière modification de la notice
03/03/2018 21:25
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