Characterization of the promoter region of the mouse Xist gene

Details

Serval ID
serval:BIB_CA4F85E2C9FF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization of the promoter region of the mouse Xist gene
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Pillet  N., Bonny  C., Schorderet  D. F.
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
12/1995
Volume
92
Number
26
Pages
12515-9
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 19
Abstract
The mouse Xist gene is expressed exclusively from the inactive X chromosome and may be implicated in initiating X inactivation. To better understand the mechanisms underlying the control of Xist expression, we investigated the upstream regulatory region of the mouse Xist promoter. A 1.2-kb upstream region of the Xist gene was sequenced and promoter activity was studied by chloramphenicol acetyltransferase (CAT) assays after transfection in murine XX and XY cell lines. The region analyzed (-1157 to +917 showed no in vitro sex-specific promoter activity. However, a minimal constitutional promoter was assigned to a region from -81 to +1, and a cis element from -41 to -15 regulates promoter activity. We showed that a nuclear factor binds to an element located at -30 to -25 (TTAAAG). A second sequence at -41 to -15 does not act as an enhancer and is unable to confer transcriptional activity to the Xist gene on its own. A third region from -82 to -41 is needed for correct expression. Deletion of the segment -441 to -231 is associated with an increase in CAT activity and may represent a silencer element.
Keywords
3T3 Cells Animals Base Sequence Binding Sites Cell Line Cell Nucleus/metabolism Chloramphenicol O-Acetyltransferase/biosynthesis *Chromosome Aberrations *Chromosome Disorders DNA-Binding Proteins/metabolism Female Humans Male Mice/*genetics Mice, Inbred Strains Molecular Sequence Data *Promoter Regions (Genetics) *RNA, Untranslated Recombinant Proteins/biosynthesis Sex Characteristics Transcription Factors/*genetics Transfection *X Chromosome
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:58
Last modification date
20/08/2019 15:45
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