Characterization of the promoter region of the mouse Xist gene

Détails

ID Serval
serval:BIB_CA4F85E2C9FF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of the promoter region of the mouse Xist gene
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Pillet  N., Bonny  C., Schorderet  D. F.
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
12/1995
Volume
92
Numéro
26
Pages
12515-9
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 19
Résumé
The mouse Xist gene is expressed exclusively from the inactive X chromosome and may be implicated in initiating X inactivation. To better understand the mechanisms underlying the control of Xist expression, we investigated the upstream regulatory region of the mouse Xist promoter. A 1.2-kb upstream region of the Xist gene was sequenced and promoter activity was studied by chloramphenicol acetyltransferase (CAT) assays after transfection in murine XX and XY cell lines. The region analyzed (-1157 to +917 showed no in vitro sex-specific promoter activity. However, a minimal constitutional promoter was assigned to a region from -81 to +1, and a cis element from -41 to -15 regulates promoter activity. We showed that a nuclear factor binds to an element located at -30 to -25 (TTAAAG). A second sequence at -41 to -15 does not act as an enhancer and is unable to confer transcriptional activity to the Xist gene on its own. A third region from -82 to -41 is needed for correct expression. Deletion of the segment -441 to -231 is associated with an increase in CAT activity and may represent a silencer element.
Mots-clé
3T3 Cells Animals Base Sequence Binding Sites Cell Line Cell Nucleus/metabolism Chloramphenicol O-Acetyltransferase/biosynthesis *Chromosome Aberrations *Chromosome Disorders DNA-Binding Proteins/metabolism Female Humans Male Mice/*genetics Mice, Inbred Strains Molecular Sequence Data *Promoter Regions (Genetics) *RNA, Untranslated Recombinant Proteins/biosynthesis Sex Characteristics Transcription Factors/*genetics Transfection *X Chromosome
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 13:58
Dernière modification de la notice
09/05/2019 1:14
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