Conditional gene targeting of the ENaC subunit genes Scnn1b and Scnn1g.

Détails

ID Serval
serval:BIB_CA2F481CDD6E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Conditional gene targeting of the ENaC subunit genes Scnn1b and Scnn1g.
Périodique
American Journal of Physiology. Renal physiology
Auteur(s)
Mérillat A.M., Charles R.P., Porret A., Maillard M., Rossier B., Beermann F., Hummler E.
ISSN
0363-6127
Statut éditorial
Publié
Date de publication
02/2009
Peer-reviewed
Oui
Volume
296
Numéro
2
Pages
F249-F256
Langue
anglais
Résumé
Epithelial sodium channels (ENaC) are members of the degenerin/ENaC superfamily of non-voltage-gated, highly amiloride-sensitive cation channels that are composed of three subunits (alpha-, beta-, and gamma-ENaC). Since complete gene inactivation of the beta- and gamma-ENaC subunit genes (Scnn1b and Scnn1g) leads to early postnatal death, we generated conditional alleles and obtained mice harboring floxed and null alleles for both gene loci. Using quantitative RT-PCR analysis, we showed that the introduction of the loxP sites did not interfere with the mRNA transcript expression level of the Scnn1b and Scnn1g gene locus, respectively. Upon a regular and salt-deficient diet, both beta- and gamma-ENaC floxed mice showed no difference in their mRNA transcript expression levels, plasma electrolytes, and aldosterone concentrations as well as weight changes compared with control animals. These mice can now be utilized to dissect the role of ENaC function in classical and nonclassic target organs/tissues.
Pubmed
Web of science
Création de la notice
18/03/2009 10:55
Dernière modification de la notice
20/08/2019 16:45
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