Simkania negevensis may produce long-lasting infections in human pneumocytes and endometrial cells.

Détails

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_C9CD458ED44A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Simkania negevensis may produce long-lasting infections in human pneumocytes and endometrial cells.
Périodique
Pathogens and disease
Auteur(s)
Vouga M., Baud D., Greub G.
ISSN
2049-632X (Electronic)
ISSN-L
2049-632X
Statut éditorial
Publié
Date de publication
01/01/2017
Peer-reviewed
Oui
Volume
75
Numéro
1
Pages
1
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Simkania negevensis is a novel Chlamydia-related bacterium and the founding member of the Simkaniaceae family within the Chlamydiales order. Little is known about the biology and pathogenesis of this bacterium. So far, S. negevensis has been considered as an amoebal symbiont, but its natural host remains unknown. Moreover, evidence of human exposition has been reported worldwide and an association with pneumonia and bronchiolitis is suspected. Here, we evaluated the ability of S. negevensis to replicate in potential environmental reservoirs, namely amoebae and arthropods, as well as in mammalian cells (Vero cells, pneumocytes and endometrial cells) and further evaluated the characteristics of its replicative vacuole. We demonstrated that S. negevensis efficiently replicates in all cell lines tested, with the shortest doubling time and an increased adhesion observed in pneumocytes. Our work highlights the specificities of the Simkania-containing vacuole compared to other Chlamydiales; contrarily to Chlamydia trachomatis, S. negevensis does not disrupt the Golgi apparatus. Importantly, our work suggests that S. negevensis infection is associated with few cytopathic effects and might persist for a prolonged time in infected cells. Further evaluation of its implication in human diseases is required; an implication in chronic or subacute respiratory infections might be suspected.

Mots-clé
Alveolar Epithelial Cells/microbiology, Amoeba/microbiology, Animals, Cercopithecus aethiops, Chlamydiaceae/classification, Chlamydiaceae/physiology, Chlamydiaceae/ultrastructure, Endometritis/microbiology, Epithelial Cells/microbiology, Female, Humans, Pneumonia/microbiology, Vero Cells, Chlamydia-related bacteria, Chlamydiales order, Simkaniaceae, free-living amoebae, intracellular bacteria, pneumonia
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2017 18:34
Dernière modification de la notice
20/08/2019 15:44
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